We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

MagZ™ Protein Purification System Technical Bulletin

Instructions for Use of Product(s)
V8830
Literature # TB336

Purification of a polyhistidine- or HQ-tagged protein that has been expressed in rabbit reticulocyte lysate is currently limited by hemoglobin in the lysate copurifying with the protein of interest. Hemoglobin copurification limits downstream applications (e.g., fluorescence-based functional assays, protein-protein interaction studies) and reduces the amount of protein purified. Thus, we developed a unique system that purifies polyhistidine- or HQ-tagged proteins expressed in rabbit reticulocyte lysate. These polyhistidine- or HQ-tagged proteins are 99% free of contaminating hemoglobin. The MagZ™ Protein Purification System provides a simple, rapid and reliable method to purify expressed polyhistidine- or HQ-tagged protein from rabbit reticulocyte lysate. Paramagnetic precharged particles can be used to isolate polyhistidine- or HQ-tagged protein from 50–500µl of TNT® Rabbit Reticulocyte Lysate with minimal copurification of hemoglobin.

Printed in USA. Revised 6/09.

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom