Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

MagZ™ Protein Purification System Technical Bulletin

Instructions for Use of Product(s)

Literature # TB336

Purification of a polyhistidine- or HQ-tagged protein that has been expressed in rabbit reticulocyte lysate is currently limited by hemoglobin in the lysate copurifying with the protein of interest. Hemoglobin copurification limits downstream applications (e.g., fluorescence-based functional assays, protein-protein interaction studies) and reduces the amount of protein purified. Thus, we developed a unique system that purifies polyhistidine- or HQ-tagged proteins expressed in rabbit reticulocyte lysate. These polyhistidine- or HQ-tagged proteins are 99% free of contaminating hemoglobin. The MagZ™ Protein Purification System provides a simple, rapid and reliable method to purify expressed polyhistidine- or HQ-tagged protein from rabbit reticulocyte lysate. Paramagnetic precharged particles can be used to isolate polyhistidine- or HQ-tagged protein from 50–500µl of TNT® Rabbit Reticulocyte Lysate with minimal copurification of hemoglobin.

Printed in USA. Revised 6/09.