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pSP-luc+NF Fusion Vector Technical Bulletin

Instructions for Use of Product(s)

Literature # TB210

The pSP-luc+NF Fusion Vector is a luciferase cassette vector containing an engineered firefly luciferase gene, luc+NF, which has been modified for maximum flexibility in constructing N-terminal fusions (NF). The pSP-luc+NF Fusion Vector is not intended for expression of luciferase in eukaryotic cells because it does not contain eukaryotic promoters, enhancers or polyadenylation signals. The luc+NF gene is positioned downstream of an SP6 promoter and a ribosome binding site. An opposing T7 promoter is located immediately downstream of luc+NF. Thus, the pSP-luc+NF Fusion Vector provides a convenient template for in vitro synthesis of both sense and anti-sense luciferase transcripts for studies involving in situ hybridization, RNA processing, RNA transfection or coupled in vitro transcription/translation and protein folding. 

Summary of Changes
At the 11/15 revision of this document, the patent information was updated to remove expired statementss and the document design was updated. 

Printed in USA. Revised 11/15.