pGEM®-7Zf(–) Vector Technical Bulletin
Instructions for Use of Product(s)
Literature # TB069
The pGEM®-7Zf(–) Vector serves as a standard cloning vector and as a template for in vitro transcription. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. The polylinker contains restriction enzyme sites that produce 5´ overhangs or blunt ends sensitive to exonuclease III), and these enzyme sites are flanked on both sides by blocks of restriction sites that generate 3´ overhangs (resistant to exonuclease III).
Printed in USA. Revised 4/17.