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pGEM®-11Zf(+) Vector Technical Bulletin

Instructions for Use of Product(s)

Literature # TB075

The pGEM®-11Zf(+) Vector can be used as a standard cloning vector and as a template for in vitro transcription. The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E.coli strains (e.g., JM109, DH5alpha®, XL1-Blue). The multiple cloning region contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII.

Printed in USA. Revised 4/17.