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PepTag® Assay Technical Bulletin

Instructions for Use of Product(s)
V5330, V5340

Literature # TB132

The PepTag® Non-Radioactive Protein Kinase Assays provide a rapid, sensitive and non-radioactive method to detect cAMP-dependent protein kinase (PKA) and protein kinase C (PKC). The PepTag® Assays use brightly colored, fluorescent peptide substrates that are highly specific for the kinases in question. Phosphorylation by PKC or PKA alters the peptide substrate net charge, allowing phosphorylated and nonphosphorylated versions of the substrate to be rapidly separated on an agarose gel. Using the PepTag® Assay, less than 10ng of kinase can be detected in under 2 hours.

Summary of Changes
The following changes were made to the 6/19 revision of this document:

  1. Edited Materials to Be Supplied by the User, Section 3.A.
  2. Updated the Related Products.

Printed in USA. Revised 6/19.