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Anti-PARP p85 Fragment pAb Technical Bulletin

Instructions for Use of Product(s)

Literature # TB273

Anti-PARP p85 Fragment pAb is a polyclonal antibody directed against the 85kDa caspase-cleaved fragment (p85) of human poly (ADP-ribose) polymerase (PARP). Anti-PARP p85 Fragment pAb specifically detects the 85kDa (apparent molecular weight) fragment of PARP that results from caspase cleavage and does not detect the 116kDa intact PARP molecule. Its specificity for the cleaved form of PARP makes this antibody a novel marker to detect apoptosis. The antibody is designed for use as an immunocytochemical/immunohistochemical marker for apoptosis in cells and tissues and can be used with standard fixatives and immunohistochemical procedures. Anti-PARP p85 Fragment pAb provides a convenient and simple method to monitor apoptosis in situ. This antibody can be used with dual labeling techniques in conjunction with TUNEL assays for a more complete analysis of the various stages of apoptosis.

Printed in USA. Revised 7/10.