Autophagy LC3 HiBiT Reporter Vector
Instructions for Use of Product(s)
Literature # 9PIGA255
The Autophagy LC3 HiBiT Reporter Vector is part of a bioluminescent, plate-based method for quantitative
assessment of autophagy. This vector can be used to stably express the reporter in a chosen cell line, and contains the following features:
- An HSV-TK promotor with PyF101 enhancer for low-level, constitutive reporter expression in mammalian cells.
- The HiBiT peptide tag for bioluminescent detection of expressed reporter protein.
- A sequence encoding the MAP1LC3B gene with a Spacer coding sequence separating the gene from the HiBiT tag.
- A kanamycin-resistance gene for selection of the plasmid in bacterial cells and a neomycin-resistance gene for selection in mammalian cells.
Printed in USA 1/18.