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Autophagy LC3 HiBiT Reporter Vector

Instructions for Use of Product(s)

Literature # 9PIGA255

The Autophagy LC3 HiBiT Reporter Vector is part of a bioluminescent, plate-based method for quantitative assessment of autophagy. This vector can be used to stably express the reporter in a chosen cell line, and contains the following features:

  • An HSV-TK promotor with PyF101 enhancer for low-level, constitutive reporter expression in mammalian cells.
  • The HiBiT peptide tag for bioluminescent detection of expressed reporter protein.
  • A sequence encoding the MAP1LC3B gene with a Spacer coding sequence separating the gene from the HiBiT tag.
  • A kanamycin-resistance gene for selection of the plasmid in bacterial cells and a neomycin-resistance gene for selection in mammalian cells.

Printed in USA 1/18.