The Promega PowerPlex® CS7 System contains seven loci: FES/FPS, LPL, F13A, F13B, Penta D, Penta E and Penta C. All of these loci, with the exception of Penta C, are used in other multiplex systems. As a result, Penta C allele frequencies are not currently available for a diverse set of races. This study presents Penta C allele frequencies for Caucasian, African American, American Indian, Asian and Hispanic populations.
Materials and Methods
PowerPlex® CS7 Systems were obtained from Promega. Extracted DNA was analyzed using the Applied Biosystems 3130xl platforms and GeneMapper® ID software, version 3.2.1. For each population 300 persons from a convenience sample were analyzed. The race of the individuals was self-identified. Results were analyzed for heterozygosity(1), Hardy Weinberg Equilibrium using the Fisher Exact Test with 10,000 shufflings(1), PIC values(2), probability of a match(3), power of discrimination(3), average probability of exclusion(4) and an estimate of the typical paternity index(5).
View a pdf version of Table 1.
Table 1. Penta C Allele Frequencies for Various Ethnic Groups.
The Asian population used in this study consisted primarily of Chinese individuals and other Asian ethnic groups. The Asian data presented are similar to Japanese Penta C frequencies(6). Note that in this table the choice of minimal allele frequencies is left to the laboratory as there are differing methods on the determination of minimal allele frequencies(7)(8). All populations were in Hardy Weinberg Equilibrium using the Fisher Exact Test. The typical paternity index ranged from 2.063 to 2.766, and the match probability ranged from 0.022 to 0.054. In conclusion, Penta C is a valid option for the expansion of current multiplex systems.