We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Restriction Enzyme Capabilities at a Glance

  • Print
  • Email

Restriction Enzyme Capabilities

A subset of Promega restriction enzymes offer capabilities that allow you to shorten your cloning workflow. These capabilities include rapid digestion of DNA in 15 minutes or less, ability to completely digest DNA directly in the GoTaq(R) Green Master Mix, and Blue/White Cloning Qualification which allows for rapid, reliable detection of transformants.

Restriction
Enzyme
Rapid Digest
Capable
GoTaq Buffer
Compatible
Blue/White
Cloning Qualified
AatII + +
AgeI + +
AluI + nt
ApaI nt + +
BamHI + + +
BglII +
ClaI + + +
DdeI + nt
DpnI + nt
DraI nt +
EcoRI + +
EcoRV + + +
HaeIII + nt
HincII nt + +
HindIII + + +
HpaI nt +
KpnI + + +
MluI nt + +
NcoI + + +
NdeI + +
NheI + +
NotI + nt +
NsiI nt + +
PstI + + +
PvuI +
PvuII + +
RsaI + nt
SacI nt nt +
SacII + +
SalI + + +
ScaI +
SgfI nt nt +
SmaI nt nt +
SpeI + nt +
SphI + + +
StuI nt +
XbaI + + +
XhoI + + +
XmaI + nt +

Rapid Digest Capable (+): Enzymes capable of digesting DNA in 15 minutes or less.

GoTaq® Buffer Compatible (+): Enzymes active and capable of digestion directly in 1X GoTaq® Green Master Mix and 1X PCR Master Mix.

Blue/White Cloning Qualified: Enzymes tested in specific subcloning reactions to assure the absence of interfering enzyme activities.

nt: Not Tested

–: Unsuccessful cutting or not qualified

Back to top

« Previous chapter: Applications and Reaction Conditions For Restriction Enzymes

Next chapter: Restriction Enzyme Reference Information »