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Protocols and Applications Guide

A complete online resource to protocols and applications for all Promega products. Get step-by-step protocols for any product, sample data, videos, citations and lab tools.

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PCR Amplification

  • Introductory animation about PCR
  • Optimization tricks
  • RT-PCR tips
  • Polymerase and transcriptase review
  • PCR & RT-PCR protocols
  • Troubleshooting
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Apoptosis

  • Gather more data from a single sample with multiplexed assays
  • Use two or more methods to confirm apoptosis
  • Detect caspase activity with anti-PARP pAb
  • Learn about the molecular mechanisms of apoptosis
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Cell Viability

  • Compare assay methods based on your needs
  • Read about factors that effect viability assay outcome
  • Learn how to multiplex cell viability with cytotoxicity or reporter assays
  • Choose an assay with the Cell Viability Assistant
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Protein Expression

  • Optimize your protein yield
  • Learn about the gold standard: TNT® Systems
  • Use the product selector tool to choose your system
  • Explore eukaryotic, mammalian and bacterial systems
  • Read about non- radioactive labeling systems
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Expression Analysis

  • Single gene or gene array systems
  • Tips for creating a ribonuclease-free environment
  • Recipes for common solutions
  • Total or polyA RNA isolation systems
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Cell Signaling

  • Animations of MAPK and PI-3K pathways
  • High or low ATP concentration kinase assays
  • Fluorescent, luminescent, radioactive and colorimetric kinase assays
  • Phosphorylation-specific antibodies for MAPK pathways
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Bioluminescent Reporters

  • pGL4: The latest generation reporter vectors
  • Dual-luciferase system: for better control
  • Live-cell substrates
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DNA Purification

  • Tips to maximize yield and quality
  • Plasmid and genomic DNA purification
  • Transfection-quality miniprep DNA
  • High-, low- and medium-throughput
  • DNA from blood
  • Automated purification at your lab bench
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Cell Imaging

  • HaloTag® Technology: One construct, multiple fluors
  • Live-cell imaging protocols
  • Rapid labeling and imaging
  • FACS®
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Protein Purification and Analysis

  • Affinity tags for purification
  • Analyzing DNA:Protein interaction
  • HaloCHIP™ System
  • Pulldown assays for protein interactions
  • Recipes for commonly used solutions
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Transfection

  • Methods for achieving transfection
  • Optimizing transfection efficiency
  • Sample transfection protocol
  • Recipes for commonly used solutions
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Cloning

  • Learn about T-Vector cloning
  • Use Flexi® Vectors for easy sequence transfer from one vector to another
  • Get technical information on restriction enzymes
  • Find out what affects PCR cloning
  • Recipes for solutions used in cloning
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DNA-Based Human Identification

  • Learn about how repetitive DNA is used for human identification applications such as forensic casework and relationship testing
  • Explore the steps required to generate a DNA profile
  • Gain information about automated key steps in the DNA-typing process
  • Discover links to Promega protocols, Profiles in DNA articles, validation guides and other resources
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Buffers for Biochemical Reactions

  • Explore how a buffer system works
  • Understand what makes a "good" buffer
  • Learn how to prepare buffers
  • Find recipes for common buffers
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Epigenetics

  • Explore epigenetic mechanisms of gene regulation
  • Discover how DNA methylation and histone modifications affect chromatin structure
  • Decipher the histone code
  • Learn about techniques to research epigenetics
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