Singapore Society of Mass Spectrometry (SSMS) Day 2019
Visit us at our booth and learn about the many solutions Promega has for you and your research!
- Exploration Theatrette
- Thursday, August 15, 2019
- 9:00 AM–5:00 PM (SGT: Singapore)
Visit us at our booth and learn about solutions for:
- Protein sample prep workflow
- Characterization of protein glycosylation
- LC/MS instrument monitoring
Scientific Poster Presentation
An Improved Immunoaffinity LC-MS/MS Workflow for the Quantitation of IgG's during Preclinical PK Studies
Michael M. Rosenblatt, Lyndsey Jager, Nidhi Nath, Marjeta Urh
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
The testing of biotherapeutic proteins (particularly IgG's) in animals is a requirement for approval by regulatory agencies, prior to treatment in humans. PK/PD studies of biotherapeutic protein metabolism have traditionally been performed using ELISA. However, the use of immunoaffinity purification coupled with LC-MS/MS has proven advantageous in part due to its ability to monitor total drug and multiple surrogate peptides. As the number of biotherapeutic proteins in the pipeline increases, so does the need for an optimized workflow. Toward this end, we have developed an optimized workflow which improves the dynamic range of the assay, decreases the digestion time, and increase the overall workflow flexibility. The assay is also compatible with automation.
Presented by Sharon Goh, Techincal Service Scientist