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10th AFSN Annual Meeting & Symposium

Come in stay a while and see what's new with Promega at 10th AFSN. Fuel up and learn about several new products at Promega Booth.

  • Beijing International Convention Center, China.
  •  Tuesday, September 4, 2018 – Friday, September 7, 2018

 Guiding your way through the forensic workflow requires energy. We can help you stay on track and escape the daily grind.We will be showcasing several new products as part of the complete workflow solution, including Spectrum CE , Spectrum Compact CE and  Maxwell RSC instruments.
You can also learn more by joining our scientific workshop or find out about our new technologies by visiting our posters.Come on in, this is the place where you want to be!


Interpretation of Y Chromosome STR and SNP Results in Forensic Casework
Presented by:
Prof. Lutz Roewer
Institute of Legal Medicine and Forensic Sciences
Berlin, Germany
Tuesday, September 4, 1:00 pm to 5:30 pm


1:30 - 1:45  Introduction

1:45 - 2:45  Current national and international guidelines on interpretation of Y-STRs and reporting to court (Germany, USA, ISFG)

2:45 - 3:45  Use of YHRD to generate match statistics (metapopulation concept, augmented counting methods, Discrete Laplace method)

3:45 - 4:00  Break

4:00 - 5:30  Casework challenge (Match probability, Mixture interpretation, Kinship analysis, Ancestry inference using Y-SNPs)

Note: We will bring casework examples, but please bring your own and enter discussion!

Please click here to register.


Maximize Information from Your Mixture Samples Using a Combined Autosomal STR and Y-STR Multiplex System
Presented by:
Ann MacPhetridge
Sr Global Commercialization Marketing Manager
Thursday, September 6, 10:50 am

The VersaPlex™ 27PY System is a 6-color, 27-loci STR system for simultaneously amplifying and genotyping 23 autosomal loci, three Y-STR loci, and Amelogenin. It is intended for forensic casework, kinship STR analysis, and databasing in regions that prefer the D6S1043 locus. In addition to D6S1043, the autosomal loci are CSF1PO, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, Penta D, and Penta E. These loci offer Probability of Identity (PI) values of 4.4 x 10-30 for four combined American populations and 6.2 x 10-27 for the Asian American population.
This 27-loci STR system will be the only D6S1043-containing multiplex that includes multiple Y-STR loci. Like the expanded CODIS-compliant kits, the DYS391 locus is included for gender-verification of Amelogenin-null samples. Additionally, two Y-STR loci with high gene diversity values , DYS570 and DYS576, are included. These three Y-STR loci allow more confident determination of the number of male contributors in complex mixtures. 
While designed for an optimal input DNA amount of 1ng, it is sensitive to low DNA input amounts. The system is robust against high concentrations of PCR inhibitors. To improve laboratory workflow efficiency, this system is designed for use with both casework samples and with reference and database samples. Direct amplification of blood or buccal samples on multiple substrate types eliminates the extraction process. To further improve efficiency, automation methods are available for multiple robotic platforms ensuring high first-pass success rates and minimizing the potential for cross-contamination.


Isolation of Purified DNA Using a Bone DNA Extraction Kit
Presented by:
Lotte Downey
Sr Global Strategic Marketing Manager

Bones constitute one of the most challenging sample types in forensic casework, and the extracted DNA is often low in quantity and degraded reducing the chances of a usable STR profile. Also, old and poor conditions of bones present additional challenges for processing. The proposed new kit containing demineralization buffer, enables an easy preprocessing method for bone samples which maximizes the chance of successful generation of STR profile.  The extracted DNA can be purified using the established DNA IQ™ chemistry both in manual and Maxwell methods for obtaining DNA that is pure and free of inhibitors. The extracted DNA was compatible with downstream applications such as PowerQuant® System and PowerPlex® Systems, thus enabling accurate human DNA quantification and STR profile generation. Examples of performance using the Bone Extraction DNA kit with a variety of bones from different ages will be shared.

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