The VersaPlex™ 27PY System is a 6-color, 27-loci STR system for simultaneously amplifying and genotyping 23 autosomal loci, three Y-STR loci, and Amelogenin. It is intended for forensic casework, kinship STR analysis, and databasing in regions that prefer the D6S1043 locus. In addition to D6S1043, the autosomal loci are CSF1PO, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, Penta D, and Penta E. These loci offer Probability of Identity (PI) values of 4.4 x 10-30 for four combined American populations and 6.2 x 10-27 for the Asian American population.
This 27-loci STR system will be the only D6S1043-containing multiplex that includes multiple Y-STR loci. Like the expanded CODIS-compliant kits, the DYS391 locus is included for gender-verification of Amelogenin-null samples. Additionally, two Y-STR loci with high gene diversity values , DYS570 and DYS576, are included. These three Y-STR loci allow more confident determination of the number of male contributors in complex mixtures.
While designed for an optimal input DNA amount of 1ng, it is sensitive to low DNA input amounts. The system is robust against high concentrations of PCR inhibitors. To improve laboratory workflow efficiency, this system is designed for use with both casework samples and with reference and database samples. Direct amplification of blood or buccal samples on multiple substrate types eliminates the extraction process. To further improve efficiency, automation methods are available for multiple robotic platforms ensuring high first-pass success rates and minimizing the potential for cross-contamination.