SLAS 2021 Resources
Welcome to our resource page for the Society for Laboratory Automation and Screening (SLAS) 2021 Digital International Conference and Exhibition. Learn about our products and technologies for high-throughput assays, including kinase target engagement, protein degradation, cell health and more!
Featured Presentation at SLAS 2021
NanoBRET™ Technology to Quantify Live Cell Target Engagement at RAS and Its Downstream Effectors
As a driver of tumorigenesis in numerous cell types, the RAS-MAPK pathway has been the subject of concerted drug discovery efforts for decades. Here we will present the use of NanoBRET™ technology to quantify live-cell target engagement to all described drug binding sites within the RAS-RAF-MEK-ERK pathway.
Presented by Matt Robers
Sr Research Scientist, Promega Corporation
Monday, January 25, 2021
7:00—7:30pm EST
Scientific Posters
Tecan the Pressure Off: A Fully Automated High-Throughput Mammalian Purification Platform Utilising Magnetic Bead Technology
Presenter by Jade Scott, GlaxoSmithKline-Stevenage, UK
Detection of Neutralizing Antibodies and Other Inhibitory Molecules of SARS-CoV 2 Spike RBD and Human ACE2 Interactions
Presenter: Hicham Zegzouti, Sr Research Scientist, Promega Corporation
View Abstract
A Homogeneous Bioluminescent Immunoassay Approach to Interrogate Cellular Signaling Pathways Activation and Deactivation
Presenter: Michael Curtin, Product Manager, Promega Corporation
View Abstract
Cellular Kinase Assays that Deliver Quantitative Compound Affinity, Occupancy, and Selectivity in Live Cells Using NanoBRET™ Technology
Presenter: Steven Edenson, Strategic Collaborations Manager, Promega Corporation
View Abstract
Learn about the newest tools to monitor protein degradation dynamics in living cells!
Download PDFTarget Engagement
Our NanoBRET™ Target Engagement Assays enable quantitative determination of compound affinity and occupancy for a target protein in live cells. The assays use a multiwell plate, addition-only format, making them scalable. This BRET method provides excellent data quality and is applicable to multiple target classes.
Protein Degradation
Study protein degradation in live cells from compound cell permeability and E3 ligase engagement to target degradation. Learn about the comprehensive selection of CRISPR-edited cell line pools and clones to facilitate studying popular protein degradation targets that Promega has to offer.
Homogeneous Immunoassays
Try the Lumit™ Immunoassays, a simple and fast alternative to conventional immunoassay methods. These assays are sensitive, have broad dynamic range and can be completed in 70 minutes or less. They are available as catalog items or through our custom order department.
Cell Health
Promega cell health assays are designed for high-throughput screening efforts and comparative analysis by multiplexing assays with other measures of cell health, such as apoptosis and autophagy. They can also be used with luciferase reporter assays or metabolic assays. Check out our complete offering of cell health screening assays.
Kinase Biology
We offer several bioluminescent assays to query the ability of compounds to bind or inhibit kinases in biochemical and cellular formats, amenable to high throughput or selectivity profiling.
Viral Research and Vaccine Development
We offer collaborative support and a broad portfolio of reagents that are used in research labs studying coronaviruses and other emerging viral diseases.
GloMax® Microplate Readers
Designed to work seamlessly with Promega assays, GloMax® microplate readers offer a range of detection options, high sensitivity and wide dynamic range. Enjoy faster, easier analysis of luminescence and fluorescence data from high-throughput screening assays—there's a GloMax® instrument for every lab!
Additional Resources
Article:
A Homogeneous Bioluminescent Immunoassay to Probe Cellular Signaling Pathway Regulation
Article:
CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide
Article:
Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement