Proteome-Targeted Drug Discovery Resources
Featured Presentation at the 2nd Proteome-Targeted Drug Discovery Summit
Design of Chemical Probes for Target Discovery and Engagement
- A photoreactive cleavable chloroalkane capture tag that can be attached to bioactive compounds
- Assess binding affinity and kinetics of target engagement via bioluminescence resonance energy transfer (BRET)
- Case studies for identification and characterization of physiologically relevant targets for bioactive compounds
Presented by Rachel Ohana, PhD
Sr. Research Scientist, Promega Corporation
Tuesday, November 17
Featured Presentation at European Protein Degradation Congress
Profiling CDK Family Mechanisms of Degradation
Exploring the correlation of real-time kinetic degradation profiles to ternary complex formation and ubiquitination.
Presented by Kristin Riching, PhD
Sr. Scientist, Promega Corporation
Study protein degradation in live cells from compound cell permeability and E3 ligase engagement to target degradation. Learn about the comprehensive selection of CRISPR-edited cell line pools and clones to facilitate studying popular protein degradation targets that Promega has to offer.
CRISPR/Cas9 knock-in tagging offers a convenient method to study endogenous biology, without the drawbacks of overexpression methods. Promega offers a comprehensive selection of CRISPR-edited cell line pools and clones for popular drug targets and cell backgrounds.
Promega offers NanoBRET™ Target Engagement Assays which use an energy transfer technique called bioluminescence resonance energy transfer (BRET) to measure molecular proximity. With these assays you are not limited by protein class or cellular background.
Try the Lumit™ Immunoassays, a simple and fast alternative to conventional immunoassay methods. These assays are sensitive, have broad dynamic range and can be completed in 30 minutes. They are available as catalog items, early-access material or through our custom order department.
We offer a number of products for high-throughput analysis of protein interactions, including pull-down assays, bioluminescence resonance energy transfer (BRET)-based assays and complementation reporter-based systems.
Targeting Proteins for Degradation: Characterizing PROTAC Kinetics and Mode of Action Using Live-Cell Assays
Quantitative Live-Cell Kinetic Degradation and Mechanistic Profiling of PROTAC Mode of Action
Studying Endogenous Protein Dynamics with CRISPR-Mediated Tagging: Understanding Your Options
A Practical Guide to CRISPR-Mediated Gene Tagging with a Bioluminescent Peptide