Protein Degradation Event Resources
Featured Presentation at Targeted Protein Degradation Summit 2020
Cellular Mechanistic Profiling of Degradation Compounds
- Several case studies to understand PROTAC mechanisms of action in different systems
- Correlation of real-time kinetic degradation profiles to ternary complex formation and ubiquitination
- Cell-based assay strategies for rank ordering compound potency and efficacy
Presented by Danette Daniels, Ph.D.
Sr Research Scientist, Promega Corporation
Wednesday, October 14
Featured Presentation at European Protein Degradation Congress
Profiling CDK Family Mechanisms of Degradation
Exploring the correlation of real-time kinetic degradation profiles to ternary complex formation and ubiquitination.
Presented by Kristin Riching, Ph.D.
Sr Scientist, Promega Corporation
Study protein degradation in live cells from compound cell permeability and E3 ligase engagement to target degradation. Learn about the comprehensive selection of CRISPR-edited cell line pools and clones to facilitate studying popular protein degradation targets that Promega has to offer.
CRISPR/Cas9 knock-in tagging offers a convenient method to study endogenous biology, without the drawbacks of overexpression methods. Promega offers a comprehensive selection of CRISPR-edited cell line pools and clones for popular drug targets and cell backgrounds.
Promega offers NanoBRET™ Target Engagement Assays which use an energy transfer technique called bioluminescence resonance energy transfer (BRET) to measure molecular proximity. With these assays you are not limited by protein class or cellular background.
Try the Lumit™ Immunoassays, a simple and fast alternative to conventional immunoassay methods. These assays are sensitive, have broad dynamic range and can be completed in 30 minutes. They are available as catalog items, early-access material or through our custom order department.
Using high-throughput assays from Promega, you can perform comparative analysis by multiplexing assays with other measures of cell health, such as apoptosis and autophagy. Check out our complete offering of cell health screening assays.
Targeting Proteins for Degradation: Characterizing PROTAC Kinetics and Mode of Action Using Live-Cell Assays
Quantitative Live-Cell Kinetic Degradation and Mechanistic Profiling of PROTAC Mode of Action
Studying Endogenous Protein Dynamics with CRISPR-Mediated Tagging: Understanding Your Options
A Practical Guide to CRISPR-Mediated Gene Tagging with a Bioluminescent Peptide