We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

HaloLink™ Protein Array Systems Technical Manual

Instructions for Use of Product(s)
G6190
Literature # TM310

The HaloLink™ Protein Array Systems provide a new way to create custom protein arrays by combining innovative HaloTag® technology, surface engineering and cell-free protein expression systems. The HaloTag® protein is a mutated hydrolase that forms a covalent bond with HaloTag® ligands. Under physiological conditions binding is rapid and highly specific, yielding a complex that is stable even under stringent conditions. Using the HaloLink™ Protein Array Systems, HaloTag® fusion proteins are expressed in a cell-free expression system and then captured on hydrogel-coated glass slides. The fusion proteins are captured directly from the expression reaction mixture without prior purification. Using this approach, multiple fusion proteins can be rapidly synthesized and immobilized in parallel on the slide surface, and a complete experiment including protein expression, custom array formation, and protein interaction analysis can be completed in less than eight hours.

Summary of Changes
The following change was made to the 12/15 revision of this document:
Updated licensing and disclaimer statements.

Printed in USA. Revised 12/15.

Download video:

Closed format: MP4
Open format: OGG
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom