We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Magne™ Protein A Beads and Magne™ Protein G Beads for Antibody Purification Technical...

Magne™ Protein A Beads and Magne™ Protein G Beads for Antibody Purification Technical Manual

Instructions for Use of Product(s)
G7471, G7472, G7473, G8781, G8782, G8783
Literature # TM371

Magne™ Protein A Beads and Magne™ Protein G Beads are magnetic affinity beads with high specificity and high capacity for binding immunoglobulins from cell culture, ascites and serum samples. The beads have excellent magnetic properties that allow rapid and efficient capture using a variety of magnetic stands. Antibody purification can be performed using a single sample or multiple samples in parallel, with sample volumes of 20µl to 50ml.
Magne™ Protein A Beads and Protein G Beads beads use a novel attachment chemistry based on the HaloTag® technology to immobilize Protein A or Protein G. The HaloTag® protein is a multi-functional protein tag that forms a covalent bond with its ligand and is used for protein expression and purification, cell imaging, protein immobilization and protein-interaction analysis. Magne™ Protein A Beads and Magne™ Protein G Beads use recombinant Protein A from Staphylococcus and Protein G from Streptococci, respectively, fused with the HaloTag® protein. The Protein A-HaloTag® (M.Wt 71kDa) or Protein G-HaloTag® (M.Wt 58kDa) fusion protein is covalently attached in an oriented fashion to a magnetic cellulose bead that is activated using a HaloTag® ligand.
The Magne™ Protein A and Protein G Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.

Summary of Changes
At the 7/15 revision, the document design was updated. 

Printed in USA. Revised 7/15.

Download video:

Closed format: MP4
Open format: OGG
Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom