PowerPlex® ESX 17 System Technical Manual

Instructions for Use of Product(s)
DC6720, DC6721
Literature # TMD024

The PowerPlex® ESX 17 System allows co-amplification and four-color fluorescent detection of seventeen loci, including D18S51, D21S11, TH01, D3S1358, Amelogenin, D16S539, D2S1338, D1S1656, D10S1248, FGA, D8S1179, vWA, D22S1045, D19S433, D12S391, D2S441 and SE33. This system includes a proprietary 5X master mix that contains hot-start Taq DNA polymerase, buffer, salts, MgCl2 and dNTPs. The PowerPlex® ESX 17 System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes: 5/16 Revision
The following changes were made to the 5/16 version of this document:
1. The kit component CC5 Internal Lane Standard 500 (CC5 ILS 500) was replaced with the WEN Internal Lane Standard 500 (WEN ILS 500). Instructions were updated throughout the document for use of this new internal lane standard.
2. The artifacts table in Section 6.L was updated.
3. Instructions for older versions of GeneScan® and Genotyper software were removed.
4. Other general updates were incorporated.

Printed in USA. Revised 5/16.