Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Target Engagement


Target engagement (TE) assays measure compound binding at select target proteins in intact cells. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.

The NanoBRET™ Target Engagement Assay

The NanoBRET™ Target Engagement Assays are live-cell binding assays that use an energy transfer technique to measure molecular proximity. The apparent affinity of test compounds is measured by competitive displacement of the NanoBRET™ tracer, which is reversibly bound to a NanoLuc® luciferase-target protein fusion in cells. Thus, when a competing compound is introduced, there is a dose-dependent decrease in the energy-transfer-based NanoBRET™ signal, which means you can quantitate the intracellular affinity between the test compound and its target protein.

Learn More

 

analyze-target-engagement-binding-affinity-13989ma-w-2

CRO Case Study

Learn how Aurelia Bioscience is using NanoBRET™ technology to speed up their drug discovery workflow.

Target Engagement Resources

Studying Drug Target Engagement with BRET

An overview of using BRET technology for target engagement in an easy-to-follow format.

Watch Video

nanobret-assay-webinar

Live-Cell Quantification of Target Engagement and Compound Residence Time Using a Novel BRET Assay

A novel BRET-based target engagement method that significantly increases the type of data from intra-cellular kinase and epigenetic targets in a live-cell context.

View Webinar

kinase-target-engagment

Finding the Nexus: A New Technique for Studying Kinase Target Engagement in Live Cells

Learn how to analyze inhibitor occupancy in living cells without disturbing the intracellular equilibrium or the cell membrane.

Read Article