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Proteasome-Glo™ Assays

A0001

Measure Chymotrypsin-, Trypsin- and Caspase-Like Activities

  • Homogeneous, luminescent enzyme-based assays
  • Simple "add-mix-measure" protocol
  • Results in 10-30 minutes after reagent addition

Choose an assay

Size

Catalog number selected: G8621

$ 340.00 Your price: Log In

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Proteasome-Glo™ Assays
Chymotrypsin-like/10ml
$ 340.00
Your price: Log In
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Fast and Simple Measurement of Protease Activity

Homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. Adding the Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminoluciferin for chymotrypsin-like, Z-LRR-aminoluciferin for trypsin-like, and Z-nLPnLD-aminoluciferin for caspase-like activity. Each luminogenic substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. The reagents are added to test samples containing proteasome enzyme that cleaves the substrates, releasing luciferin, which is consumed by luciferase, producing "glow-type" luminescence correlating to enzyme activity or inhibition.

 The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays in an enzyme-based format (each of these three assays also is available separately). The simplified method minimizes handling steps and makes the assays amenable to automation. The luminescent assay format avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assays are miniaturizable to 384-well format.

5855MA-W
The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.
Luminescence is proportional to proteasome concentration.
5862MA-W
Luminescent proteasome assays are more sensitive than fluorescent proteasome assays.
5994MA-W

 Number of assays per plate

Product Cat.# # Assays Volume/Assay Plate Size
Proteasome-Glo™ Chymotrypsin-Like Assay
G8621 100 100µl 96-well
400 25µl 384-well
G8622 500 100µl 96-well
2,000 25µl 384-well
Proteasome-Glo™ Trypsin-Like Assay G8631 100 100µl 96-well
400 25µl 384-well
G8632 500 100µl 96-well
2,000 25µl 384-well
Proteasome-Glo™ Caspase-Like Assay
G8641 100 100µl 96-well
400 25µl 384-well
G8642 500 100µl 96-well
2,000 25µl 384-well
Proteasome-Glo™ 3-Substrate System
G8531 100* 100µl 96-well
400* 25µl 384-well
G8532 500* 100µl 96-well
2,000* 25µl 384-well

 *For each of the 3 systems.

Protocols

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854A 1 x 10ml

Suc-LLVY-Glo™ Substrate

G856C 1 x 50μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854B 1 x 50ml

Suc-LLVY-Glo™ Substrate

G856D 1 x 250μl

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854A 1 x 10ml

Z-LRR-Glo™ Substrate

G855A 1 x 100μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854B 1 x 50ml

Z-LRR-Glo™ Substrate

G855B 1 x 500μl

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854A 1 x 10ml

Z-nLPnLD-Glo™ Substrate

G857A 1 x 50μl

Luciferin Detection Reagent

V859A 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Buffer

G854B 1 x 50ml

Z-nLPnLD-Glo™ Substrate

G857B 1 x 250μl

Luciferin Detection Reagent

V859B 1 x 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Chymotrypsin-Like Assay

G8621 1 x 10ml

Proteasome-Glo™ Trypsin-Like Assay

G8631 1 x 10ml

Proteasome-Glo™ Caspase-Like Assay

G8641 1 x 10ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: 50ml Change Configuration

What's in the box?

Item Part # Size

Proteasome-Glo™ Chymotrypsin-Like Assay

G8622 1 x 50ml

Proteasome-Glo™ Trypsin-Like Assay

G8632 1 x 50ml

Proteasome-Glo™ Caspase-Like Assay

G8642 1 x 50ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Resources

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Small Molecule Drug Discovery Workflow

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