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DPPIV-Glo™ Protease Assay

A0002

Homogeneous, Luminescent Assay that Measures Dipeptidyl Peptidase IV Activity

  • Simple "add-mix-measure" protocol
  • Results in as little as 30 minutes after reagent addition
  • Signal stability allows batch processing

Size

Catalog number selected: G8350

$ 340.00 Your price: Log In

DPPIV-Glo™ Protease Assay
10ml
$ 340.00
Your price: Log In
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Simple, Sensitive Assay for DPPIV Activity

The DPPIV-Glo™ Assay measures dipeptidyl peptidase IV (DPPIV) activity can be used to determine DPPIV enzyme activity or perform kinetic studies of DPPIV inhibitors. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with l-proline or l-alanine at the penultimate position. The assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a single DPPIV-Glo™ Reagent in an "add-mix-measure" format results in DPPIV cleavage of the substrate and generation of a "glow-type" luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of DPPIV activity present. The assay is designed for use with purified enzyme preparations.

  • Simplified method is highly amenable to automation.
  • More sensitive than fluorescent-based DPPIV assays; the luminescent assay avoids inherent fluorescent background signals and thus provides excellent signal-to-background readings. The assay is linear over more than 3 logs of DPPIV concentration and can detect less than 1pg/ml enzyme.
  • Maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition and, unlike fluorescent assays, is not dependent on accumulation of cleaved product.
  • Amenable to batch processing with a stable signal that allows plates to be read over an extended period of time.
4794MA-W
The proluminescent substrate containing the Gly-Pro sequence recognized by DPPIV.

Sensitivity of the DPPIV-Glo™ Protease Assay compared to a fluorescent assay.

4799MA-W

Number of Assays Per Plate

Product Cat.# # Assays Volume/Assay Plate Size
DPPIV-Glo™ Assay
G8350 100 100µl 96-well
200 50µl 384-well
400 25µl 384-well
G8351 500 100µl 96-well
1,000 50µl 384-well
2,000 25µl 384-well

Specifications

You are viewing: G8350 Change Configuration

What's in the box?

Item Part # Size

DPPIV-Glo™ Buffer

G836A 1 × 10ml

DPPIV-Glo™ Substrate

G837A 1 × 26.5μl

Luciferin Detection Reagent

V859A 1 × 1 each

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

Specifications

You are viewing: G8351 Change Configuration

What's in the box?

Item Part # Size

DPPIV-Glo™ Buffer

G836B 1 × 50ml

DPPIV-Glo™ Substrate

G837B 1 × 115.2μl

Luciferin Detection Reagent

V859B 1 × 1 each

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.

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