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The DPPIV-Glo™ Assay measures dipeptidyl peptidase IV (DPPIV) activity can be used to determine DPPIV enzyme activity or perform kinetic studies of DPPIV inhibitors. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with l-proline or l-alanine at the penultimate position. The assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a single DPPIV-Glo™ Reagent in an "add-mix-measure" format results in DPPIV cleavage of the substrate and generation of a "glow-type" luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of DPPIV activity present. The assay is designed for use with purified enzyme preparations.
Number of Assays Per Plate
DPPIV-Glo™ Protease Assay Technical Bulletin
PDF (397 KB)
Luciferin Detection Reagent
PDF (0 B)
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See Protocol for storage recommendations.
U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.
Homogeneous, luminescent assays that measure protease activities in cultured cells.
G8660, G8661, G8662, G8760, G8761, G8860, G8861, G1180, G1200
Measure chymotrypsin-, trypsin- and caspase-like activities.
G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532
Homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities.
Detect and measure protease activities.
For use with discontinued GloMax® 96 Microplate Luminometer instrument.
E6531, E4822, E4861
For use with the discontinued GloMax®-Multi Jr Single-Tube Multimode Reader.
E6071, E6072, E6073, E6074, E6075, E6076, E6077, E6078, E6079, E6094, E6081, E6091, E6092, E6093, E6095, E2821, E2851
High-performance multimode system for cell health and metabolism, reporter gene activity and NGS applications.
Easy-to-use, flexible multimode detection system.
Ultrasensitive single tube luminometer.
E5311, E5321, E5331
Dedicated microplate luminometer.
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