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Assay Development and Services

The Promega Assay Development and Services team was established in 2009 to support complete custom solutions for biochemical and cell-based assays. Speed up your workflow with access to our latest technologies, R&D expertise and our commitment to high quality standards. We provide all the post-delivery support to ensure your assay works in your hands. 

In addition to our custom-developed assays and consulting services, we offer prebuilt materials if you choose to design your own assay. Our experience in custom assay development covers a breadth of research areas, such as targeted protein degradation, CRISPR gene editing, bioassays, kinase biology, and more.

Why Partner with Promega Assay Development?

proven experience
automated format options
primary manufacturer
application-specific designs
protein degradation crispr knock-in

Protein Degradation/CRISPR Knock-in

The combination of CRISPR/Cas9 knock-in and live-cell bioluminescent detection methods offers a novel approach to study endogenous biology. Changes in protein abundance can be monitored in real time to better understand cellular protein dynamics. 

Services include:

  • Custom knock-in cell line development
  • Protein degradation profiling with optional cell viability monitoring
  • View our current list of available cell line pools and clones

assay services for biologics

Biologics

Promega offers a broad portfolio of functional reporter bioassays and primary cell-based target cell killing assays. Together with our bioassay development and qualification services, we can help accelerate your biologics drug discovery and development workflow.

Services include:

  • Bioassay design and development
  • Assay optimization using client antibody drugs
  • Stable cell line development
  • Manufacture of bioassay cells in Thaw and Use format
  • Bioassay and reagent qualification services
nanobret target engagement assays

NanoBRET™ Target Engagement Assays

The NanoBRET™ Target Engagement Assays are live-cell binding assays that use an energy transfer technique to measure molecular proximity. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.

Services include:

  • Tracer synthesis (from total synthesis to dye conjugation of target molecules)
  • Assay development for novel protein targets 
  • Compound profiling of existing targets (e.g., CRBN, VHL)
  • Selectivity profiling of drug candidates against >200 kinase proteins and compound dose-response follow-ups
protein interactions ppi

Protein:Protein Interaction (PPI) Assays

The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in vivo using BRET (NanoBRET™) or complementation reporter-based (NanoBiT®) methods. These assays enable detection of protein interactions at endogenous expression levels in high-throughput, multiwell plate formats.

Services include:

  • NanoBRET™ PPI Assays: Vectors, assay optimization, stable cell line development, compound testing 
  • NanoBiT® PPI Assays: Vectors, assay optimization, stable cell line development, compound testing 
lumit immunoassays

Lumit™ Technology for Immunoassays

Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods, including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 30 minutes. 

Services include:

  • SmBiT® and LgBiT® antibody labeling for X-Y antibodies that can be used to determine the optimal Ab pair for an immunoassay
  • Bulk labeling of selected antibodies from 1mg to 10mg
  • Testing primary Ab pairs with our pre-labeled secondary Ab detection system
  • Custom immunoassay development
reporter gene assays

Reporter Gene Assays

Reporter gene assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of assay products and tools for your research.

Services include:

  • Reporter vectors
  • Assay optimization
  • Stable cell line development
  • Compound testing  

Discuss your assay development requirements with our scientists.

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