Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

ADME/Toxicity

A critical stage in the drug discovery process is studying the absorption, distribution, metabolism and excretion (ADME) of drug candidates. These kinetic studies, together with screening for drug toxicity, help to build a pharmacological profile of the drug.

Case Study: ADME/Tox Screening in 3D Culture

Learn how Swansea University Medical School is using 3D liver cell models to study cell viability and metabolic activity for nanomaterial hazard testing.

Assays for ADME/Toxicity


Bioluminescence-based assays offer considerable time savings in drug ADME/Tox screening, compared to conventional methods. Background is minimal compared to fluorescence-based assays, since there is no naturally occurring source of bioluminescence in most cell lines. Cell-based CYP metabolism assays can be adapted to 3D cell culture with optimized protocols.


View Products

ADME/Toxicity Resources

adme-tox-luminescent-viability-assays-article

Luminescent Viability Assays for Magnetically Bioprinted Hepatocyte Spheroids

Print hepatocyte spheroids with magnetic 3D bioprinting, then assay the metabolic activity (CYP3A4) and viability of these spheroids using the P450-Glo™ and CellTiter-Glo® luminescent assays.

Read Article

hepatotoxicity-3d-cell-culture

An Automated Multiplexed Hepatotoxicity and CYP Induction Assay Using HepaRG Cells in 2D and 3D

This study introduces novel methods to evaluate drug metabolism and hepatotoxicity in both monolayer and 3D cultures.

Read Article

cytochrome-p450-activity-3d-hapatocytes

Screening of Cytochrome P450 Activity in Magnetic 3D Hepatocytes

A review of a research publication demonstrating the use of luminescent assays to measure cytochrome P450 activity in magnetic hepatocytes.

Read Article