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ProNex® Size-Selective Purification System

Superior Size Selection and Recovery for NGS and PCR

  • High specificity of size selection with low carryover of unwanted DNA
  • Exceptional DNA recovery
  • Fast magnetic response time and low viscosity for accurate pipetting

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Catalog number selected: NG2001

$ 300.00
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ProNex® Size-Selective Purification System
10ml
$ 300.00
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NGS size selection beads

Efficient, Accurate Size Selection

The ProNex® Size-Selective Purification System enables the rapid and efficient magnetic resin-based purification of double-stranded DNA (dsDNA) for next-generation sequencing (NGS), polymerase chain reaction (PCR) and general molecular biology applications. The ProNex® System allows users to select the desired size of purified dsDNA fragments, from 100bp to 750bp. The novel reagent formulation provides significantly improved selectivity, reproducibility and yield relative to traditional dsDNA purification methods. In addition, the ProNex® System can be used in both manual and automated high-throughput workflows.

ProNex Size-Selective DNA Purification System

Control Your DNA Size

By adding varying ratios of ProNex® Size Selection Chemistry to your sample, you can control what size DNA is retained or eliminated. This enables NGS library and PCR fragment cleanup, as well as dual size selection of libraries.

DNA Purification and DNA Size Selection

Superior DNA Recovery

ProNex NGS Library Prep versus AMPure DNA Recovery

Serial buffer exchange/cleanup purification comparison between ProNex® Size-Selective Purification System and AMPure® XP beads. Five sequential cleanup rounds (AMPure® XP = 1.8X ratio, ProNex® = 3X ratio) were performed on a 200bp DNA step ladder. Ratios designed to retain all fragments. N=8 replicates per sample.

Sensitive, Accurate Single-Size Cleanup

DNA Size Selection Chemistry:Sample Ratios
Size selection of DNA with varying ProNex® chemistry:sample ratios.

Easy to Pipette with Fast Bead Response

Significantly Lower Viscosity—No Sticking to Tips

Lower Viscosity with ProNex NGS Library Prep

ProNex® beads (left) have significantly lower viscosity than AMPure® XP beads (right), making them far easier to pipette accurately and easily, increasing reproducibility of size selection and recovery.

5X Greater Bead Response Time—See the Difference

NGS Library Prep Greater Bead Response Time

ProNex® beads (bottom) have a magnet response time that is ~5X faster than AMPure® XP beads (top), ensuring tighter bead pelleting and greater confidence in sample handling.

More Accurate Dual Library Size Selection

Getting the most sequencing data for your money requires precise size selection of your library. Elimination of both small and large fragments outside the desired range is critical to avoid wasting money on unusable reads. The ProNex® Size Selective Purification System not only removes more low- and high-molecular weight DNA than existing methods but also retains more of the target fragment sizes you really want.

ProNex NGS Library v AMPure DNA Size Selection Panel A
ProNex NGS Library v AMPure DNA Size Selection Panel B
AMPure v ProNex NGS Library DNA Size Selection Panel C
DNA Size Selection ProNex NGS Library v AMPure Panel D

E. coli libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, using starting input values of 1,000ng, 100ng, 10ng and 1ng. Libraries were size selected with either AMPure® XP beads or the ProNex® Size-Selective Purification System. Libraries were centered at 300bp, using recommended library kit manufacturer’s recommendations for size selection with AMPure® XP beads and Promega’s guidelines for the ProNex® Size-Selective Purification System. Post-PCR libraries are shown. Libraries generated using the ProNex® System significantly reduced high-molecular weight DNA carryover and produced tighter size distribution.

Significantly Higher Library Yields

DNA Purification and Higher Library Yields

Total library yield comparison between ProNex® Size-Selective Purification System and AMPure® XP beads. E. coli libraries were created using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, using starting input values of 1,000ng, 100ng, 10ng and 1ng. Libraries were size selected with either AMPure® XP beads or the ProNex® Size-Selective Purification System. Libraries were centered at 300bp, using recommended library kit manufacturer’s recommendations for size selection with AMPure® XP beads and Promega’s guidelines for the ProNex® Size-Selective Purification System. Total yield of post size-selection libraries are shown.

Better Library Prep = The Sequencing Data You Want

ProNex® Chemistry delivers tighter distribution of desired fragment sizes with minimal fragments outside of your chosen range. A more narrow distribution means fewer wasted reads and more efficient sequencing.

Comparison of Library Fragment Size Distributions

ProNex NGS Library Preparation versus AMPure 1000ng
ProNex NGS Library Prep versus AMPure 100ng
ProNex NGS Library Preparation versus AMPure 10ng
ProNex NGS Library Prep versus AMPure 1ng

Comparison of Sequencing Metrics (1000ng Sample Inputs)

NGS Sequencing Metrics ProNex versus AMPure
Next Generation Sequencing Metrics Comparison

Specifications

You are viewing: NG2001 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ProNex® Size-Selective Chemistry

NG103A 1 × 10ml

Wash Buffer

NG105A 1 × 15ml View Product

Elution Buffer (10mM Tris [pH 8.5])

NG116A 1 × 10ml

SDS

Choose language:

Download SDSPDF (86 KB) – English (United States)

Certificate of Analysis

See all certificates for NG2001

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Specifications

You are viewing: NG2002 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ProNex® Size-Selective Chemistry

NG103B 1 × 125ml

Wash Buffer

NG105B 1 × 180ml View Product

Elution Buffer (10mM Tris [pH 8.5])

NG116B 1 × 125ml

SDS

Choose language:

Download SDSPDF (86 KB) – English (United States)

Certificate of Analysis

See all certificates for NG2002

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Specifications

You are viewing: NG2003 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

ProNex® Size-Selective Chemistry

NG103B 4 × 125ml

Wash Buffer

NG1051 2 × 340ml View Product

Elution Buffer (10mM Tris [pH 8.5])

NG116B 4 × 125ml

SDS

Choose language:

Download SDSPDF (86 KB) – English (United States)

Certificate of Analysis

See all certificates for NG2003

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

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