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ProNex® NGS Library Quant Kit

ng1201

Accurate, Reproducible Quantitation for NGS libraries

  • Measure intact, functional library concentration
  • BRYT Green® dye-based qPCR system for maximum reproducibility
  • Ideal for multiplexed pooling of Illumina libraries

Size

Catalog number selected: NG1201

$ 650.00
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ProNex® NGS Library Quant Kit
500 reactions
$ 650.00
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NGS size selection beads

The Most Consistent NGS Library Quantitation Kit

The ProNex® NGS Library Quant Kit contains reagents to determine the concentration of next generation sequencing libraries compatible with Illumina platforms using qPCR. Data generated using the ProNex® NGS Library Quant Kit can be used to normalize the DNA concentration for sequencing libraries to the desired concentration prior to multiplex pooling of samples. This is the most consistent NGS library quantitation kit you can get.

Superior Precision and Reproducibility

More consistent cluster densities mean more reproducible read depths to enable predictable sequencing coverage, confident mutation detection and lower overall sequencing costs.
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Illumina NGS libraries were quantified using either the ProNex® NGS Library Quant Kit or the KAPA Library Quantification Kit for Illumina (Kapa BioSystems). The determined concentrations were used to normalize and pool libraries (n=10 pools) prior to sequencing on an Illumina MiSeq instrument. Targeted cluster density was 1000K/mm2. Cluster density was measured for all sequencing runs, showing significantly improved reproducibility when samples were quantified with the ProNex® system.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

ProNex® NGS Library Quant 2X Master Mix

NG115A 5 × 1ml

ProNex® NGS Library Quant 10X Primer Mix

NG803A 1 × 1ml

ProNex® NGS Quant DNA Standard

NG104A 1 × 25μl

ProNex® NGS Library Dilution Buffer

NG146A 2 × 150ml

Nuclease-Free Water

P119A 2 × 1,250μl View Product

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

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