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T7 RiboMAX™ Express RNAi System

Produces Milligam Amounts of RNA for RNA Interference Experiments

  • siRNA duplexes for mammalian RNAi
  • Long dsRNA for nonmammalian RNAi
  • Produces milligram amounts of double-stranded RNA in 30 minutes

Size

Catalog number selected: P1700

$ 523.00 Your price: Log In

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T7 RiboMAX™ Express RNAi System
50 × 20µl reactions
$ 523.00
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The T7 RiboMAX™ Express RNAi System is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and nonmammalian systems.

The T7 RiboMAX™ Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21bp for use in mammalian systems. siRNAs synthesized in vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells.

In addition, the T7 RiboMAX™ Express RNAi System can be used for the synthesis of dsRNA molecules of approximately 200bp or greater, which can be applied to nonmammalian systems. Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications.


  • Save Time: The T7 RiboMAX™ Express RNAi System produces milligram amounts of RNA in as little as 30 minutes.
  • Minimize Pipetting Errors: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time.

  1. Yu, J.-H. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 6047–52.
  2. Donze, O. and Picard, D. (2002) Nucl. Acids Res. 20, e46.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

RNase A Solution

A797D 1 x 200μl4mg/ml View Product

Sodium Acetate, 3.0M (pH 5.2)

P135A 1 x 1ml

Enzyme Mix, T7 Express

P132A 1 x 100μl

Nuclease-Free Water

P119A 1 x 1,250μl View Product

RiboMAX™ Express T7 2X Buffer

P164A 1 x 500μl

pGEM® Express Positive Control Template

P256A 1 x 5μg

RQ1 RNase-Free DNase

M610C 1 x 110u View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

This product is covered under license from Carnegie Institution of Washington under U.S. Pat. Nos. 6,506,559, 7,538,095, 7,560,438, Australian Pat. No. 743798 and other patents pending. Commercial use of this product will require a separate license from Carnegie.

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