We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

SV 96 Total RNA Isolation System

Z3500

Purifies Total RNA From 96 Samples in Under an Hour

  • Vacuum-based method. No centrifugation required
  • Plate design prevents cross-contamination during sample processing
  • Designed for both manual and automated RNA purification

Size

Catalog number selected: Z3500

$ 350.00 Your price: Log In

Add to Helix Add to Helix
SV 96 Total RNA Isolation System
1 × 96 each
$ 350.00
Your price: Log In
Change Configuration

Manual or High-Throughput RNA Purification. No Phenol, No Ethanol, No Fuss.

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.

Protocols are available for Beckman Coulter and PerkinElmer instruments.

  • Tested to ensure that purified RNA performs optimally in RT-PCR.
  • Novel vacuum manifold eliminates waste handling. Novel plate design prevents cross-contamination during sample processing.
  • Validated automated methods available at: www.promega.com/automethods/
  • Learn more about our custom options for this product at: www.promega.com/custom/

The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

For automated total RNA purification using the Biomek® 2000 Laboratory Automation Workstation, the Vacuum Manifold (Beckman Coulter Part# 609670) and Collar (Beckman Coulter Part# 609597) are required. See the Automated Protocol, #EP003, for additional information on automated RNA purification.

Protocols

Specifications

You are viewing: 1 × 96 each Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Binding Plate

A227A 1 x 1 plate View Product

Elution Plate

A228A 1 x 1 plate

Plate Sealers

A230A 1 x 3 each

Nuclease-Free Water

P119E 1 x 13ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 x 50ml

RNA Wash Solution (RWA)

Z3091 1 x 58.8ml

DNase Stop Solution (DSA)

Z312C 1 x 13.25ml

Yellow Core Buffer

Z317A 1 x 2.5ml

MnCl2, 0.09M

Z318B 1 x 750μl

DNase I (lyophilized)

Z358A 1 x 1 vial

Beta-Mercaptoethanol

Z523A 1 x 2ml48.7%

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Specifications

You are viewing: 5 × 96 each Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Binding Plate

A227A 5 x 1 plate View Product

Elution Plate

A228A 5 x 1 plate

Plate Sealers

A230A 2 x 3 each

Nuclease-Free Water

P119E 5 x 13ml View Product

RNA Lysis Buffer (RLA)

Z3051 2 x 50ml

RNA Wash Solution (RWA)

Z3091 5 x 58.8ml

DNase Stop Solution (DSA)

Z312C 5 x 13.25ml

Yellow Core Buffer

Z317A 5 x 2.5ml

MnCl2, 0.09M

Z318B 5 x 750μl

DNase I (lyophilized)

Z358A 5 x 1 vial

Beta-Mercaptoethanol

Z523A 2 x 2ml48.7%

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Resources

workflow-background

RNA Analysis Workflow

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom