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ReliaPrep™ RNA Miniprep Systems

Z6010

Efficiently Purify RNA from as Few as 100 Cells or 0.25mg Tissue

  • Simple, 30-minute protocol
  • Elutes in small volume, generating highly concentrated RNA
  • No phenol/chloroform or ß-mercaptoethanol required

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Catalog number selected: Z6010

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ReliaPrep™ RNA Miniprep Systems
Cells/10 preps
$ 78.00
Your price: Log In
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Recover Concentrated, Pure RNA from Precious Cell and Tissue Samples

Precious samples, sensitive assays, low copy messages – the effectiveness of RNA purification sets the stage for downstream results.

The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). 

Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or 0.25 to 20mg of tissue can be processed per purification. The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.

  • Small sample size and low elution volume allows conservation of precious samples.
  • Maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets.
  • Usable, concentrated RNA is eluted in as little as 7µl. No need to further concentrate samples.
  • Rapid protocol and provided DNase reagents streamline laboratory processes.
  • Purified RNA is suitable for many common downstream assays.
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RNA from Cells
RNA fromTissues

Higher RNA Yield

The data to the right show that the ReliaPrep™ Cell RNA Miniprep System efficiently isolates RNA from 100 to 1 x 106 HeLa cells with a higher RNA yield than the leading competitor.  Below, the RNA yield from 100,000 cells of 4 different cell lines. More data on performance of the ReliaPrep™ Systems is available in this Application Note.

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Yield and Purity of RNA from Tissues

This data shows typical RNA yields from fibrous and nonfibrous mouse tissues using the ReliaPrep™ RNA Tissue Miniprep System. RNA was isolated from 10mg samples of various mouse tissues. Yield and purity were determined by absorbance spectroscopy using a Nanodrop® 1000 instrument.

More data illustrating use of the ReliaPrep™ RNA Tissue Miniprep System is available in this Application Note.

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Protocols

Specifications

You are viewing: Z6010 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 1 × 75μl
Column Wash Solution (CWE) A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

BL Buffer

Z103A 2 × 3.25ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6011 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130A 2 × 50/pk
ReliaPrep™ Minicolumns A207B 1 × 50/pack

1-Thioglycerol

A208B 1 × 900μl
Column Wash Solution (CWE) A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

BL Buffer

Z103B 1 × 32.5ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6012 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130B 2 × 250/pk
ReliaPrep™ Minicolumns A207B 5 × 50/pack

1-Thioglycerol

A208B 2 × 900μl
Column Wash Solution (CWE) A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product
BL Buffer Z103C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6110 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130C 2 × 10/pk
ReliaPrep™ Minicolumns A207A 1 × 10/pack
1-Thioglycerol A208A 2 × 75μl
Column Wash Solution (CWE) A254A 1 × 1ml

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Lysis Buffer (LBA)

Z106A 1 × 6ml

Elution Tubes

Z200B 1 × 25/pk

RNA Wash Solution (RWA)

Z309B 1 × 11.8ml View Product
Yellow Core Buffer Z317B 1 × 300μl

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358B 1 × 1 vial

RNA Dilution Buffer (RDB)

Z372C 1 × 9ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6111 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130A 2 × 50/pk
ReliaPrep™ Minicolumns A207B 1 × 50/pack

1-Thioglycerol

A208B 2 × 900μl
Column Wash Solution (CWE) A254B 1 × 5ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106B 1 × 30ml

Elution Tubes

Z200C 1 × 50/pk
RNA Wash Solution (RWA) Z309E 1 × 35ml View Product

Yellow Core Buffer

Z317A 1 × 2.5ml

MnCl2, 0.09M

Z318A 1 × 250μl

DNase I (lyophilized)

Z358A 1 × 1 vial

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: Z6112 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130B 2 × 250/pk
ReliaPrep™ Minicolumns A207B 5 × 50/pack

1-Thioglycerol

A208B 4 × 900μl
Column Wash Solution (CWE) A254C 1 × 24ml

Nuclease-Free Water

P119E 1 × 13ml View Product

Lysis Buffer (LBA)

Z106C 1 × 150ml

Elution Tubes

Z200C 5 × 50/pk

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

Yellow Core Buffer

Z317C 1 × 11ml

MnCl2, 0.09M

Z318B 2 × 750μl

DNase I (lyophilized)

Z358A 3 × 1 vial

RNA Dilution Buffer (RDB)

Z372B 1 × 220ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

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RNA Analysis Workflow

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