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ReliaPrep™ FFPE Total RNA Miniprep System

Z1001

Intact, Amplifiable Total RNA from FFPE Tissues

  • Minimal preparation time
  • Safely deparaffinize without harsh organic solvents
  • No overnight digestion

Size

Catalog number selected: Z1001

$ 130.00 Your price: Log In

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ReliaPrep™ FFPE Total RNA Miniprep System
10 reactions
$ 130.00
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32462420-gDNA-ffpe-overview

More Functional RNA Yield from FFPE Samples

The ability to extract RNA from FFPE samples allows correlation of disease state and tissue morphology with gene expression. Extraction of RNA from FFPE tissues is challenging because the formalin fixation process cross-links proteins and nucleic acids and introduces modifications that can inhibit subsequent analysis of RNA isolated from FFPE tissues.

The ReliaPrep™ FFPE Total RNA Miniprep System optimizes lysis conditions to reverse these RNA modifications without the need for overnight digestion. The system also uses a deparaffinization method that does not rely on xylene or other organic solvents. By eliminating the washing steps involved in xylene deparaffinization, this method can help retain small RNA fragments.

The protocol is complete in 1.5 hours with minimal hands-on time.

ReliaPrep™ FFPE Miniprep Systems help preserve RNA integrity

In this experiment, 5µl purified RNA was used as template in a GoScript™ Reverse Transcription System reaction and 5µl of the cDNA product was used as template for the multiplex PCR. Fragments from 80bp-290bp were detected.

10141TB-W

Purified FFPE DNA is compatible with sensitive assays

Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641). 

10187ta-W

Simple Protocol

10146ME-W

Customer Data

1098TA-web-positionA
 

Sample Type: Rat kidney, 2 years old. Four 10µm FFPE sections were purified together (total amount = 40µm) and diluted in 40µl. Eight microliters were used for gel electrophoresis.

10398TA-web-positionB
 

Sample Type: Human liver tumor, fixed less than 1 year. One microliter out of 40µl total dilution of RNA was used for the gel.

1098TA-web-positionC
 

Sample Type: Human kidney tumor, 2 months old. Two samples were extracted using the total RNA kit. Total dilution volume was 30µl, and the loading volume for agarose gel electrophoresis was 15µl. Purity: A260 / A280 ~2.0 A260 / A230 ~2.1 Concentration: 200ng/µl.

Protocols

Specifications

You are viewing: 10 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130C 1 x 10/pk

Mineral Oil

A244A 1 x 5ml

ReliaPrep™ FFPE Binding Columns

A247A 1 x 10/pk

Proteinase K (PK) Solution

A505A 1 x 250μl View Product

Nuclease-Free Water

P119A 1 x 1,250μl View Product

Lysis Buffer (LBA)

Z101A 1 x 1ml

DNase Buffer

Z102A 1 x 70μl

BL Buffer

Z103A 1 x 3.25ml

Wash Solution

Z104A 1 x 3ml

Elution Tubes

Z200A 2 x 5/pk

MnCl2, 0.09M

Z318A 1 x 250μl

DNase I (lyophilized)

Z358A 1 x 1 vial

Blue Dye

MC122A 1 x 100μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: 100 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130A 2 x 50/pk

Mineral Oil

A244B 1 x 50ml

ReliaPrep™ FFPE Binding Columns

A247B 2 x 50/pk

Proteinase K (PK) Solution

A505B 1 x 1.1ml View Product

Nuclease-Free Water

P119E 1 x 13ml View Product

Lysis Buffer (LBA)

Z101B 1 x 30ml

DNase Buffer

Z102B 1 x 700μl

BL Buffer

Z103B 1 x 32.5ml

Wash Solution

Z104B 1 x 30ml

Elution Tubes

Z200C 2 x 50/pk

MnCl2, 0.09M

Z318B 2 x 750μl

DNase I (lyophilized)

Z358A 4 x 1 vial

Blue Dye

MC122A 1 x 100μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Resources

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RNA Analysis Workflow

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