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PureYield™ RNA Midiprep System

High Yields of Total RNA Without Using Organic Solvents

  • Purifies total RNA directly from cultured cells, bacteria, blood, yeast, plants and other sample types
  • Undetectable genomic DNA contamination
  • Rapid, safe and efficient RNA purification method

Size

Catalog number selected: Z3740

$ 143.00 Your price: Log In

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PureYield™ RNA Midiprep System
10 preps
$ 143.00
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Pure Total RNA. No Organic Solvents or Ethanol Precipitation

The PureYield™ RNA Midiprep System allows rapid purification of total RNA with undetectable levels of genomic DNA contamination without using DNase. A novel combination of reagents, membranes and protocol leads to yields of up to 1mg of total RNA without organic solvents, protease digestions or alcohol precipitations. One kit can be used to isolate pure total RNA from a wide variety of sample types, such as tissues, cultured cells, bacteria, yeast, plants and blood. The protocol also can be adapted for other sample types.

Other commonly used RNA isolation methods provide total RNA that is contaminated with genomic DNA  that interferes with sensitive methods, such as real-time RT-PCR and microarray analysis. The PureYield™ RNA Midiprep System avoids this problem by selectively removing genomic DNA prior to total RNA purification. The eluted total RNA is free of detectable DNA and ready for use in sensitive downstream applications.

The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold) formats.

  • Purified total RNA with undetectable genomic DNA contamination improves results in downstream applications.
  • Purifying total RNA without the use of DNase treatment reduces steps during purification and in downstream applications.

Process Multiple Sample Types

Blood
Sample TypePromegaExternalComments
Blood and blood cells
A general protocol for total RNA isolation from blood cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279. Additional resources are listed below.
Human blood
Yes
A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource: Brisco, P. et al. (2006) Promega Notes 92, 14–9
Mammalian Tissues
Sample TypePromegaExternalComments
Mammalian tissues
A general protocol for total RNA isolation from tissue is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below.
Rat liver
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat lung
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat kidney
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat spleen
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat brain
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat heart
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Rat muscle
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Bovine liver
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Bovine spleen
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Bovine heart
Yes
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Plant Tissues
Sample TypePromegaExternalComments
Plant tissues
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Canola leaf
Yes
Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Corn/maize leaf, stem and silk tissue
Yes
Yes
Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Cultured Cells
Sample TypePromegaExternalComments
Cultured cells
Yes
A general protocol for total RNA isolation from mammalian cultured cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Bacteria
Sample TypePromegaExternalComments
Bacteria
A general protocol for total RNA isolation from Gram-positive and Gram-negative bacteria is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below.
Escherichia coli
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Bacillus subtilis
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Bacillus sphaericus
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Bacillus globigii
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Artherobacter luteus
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Micrococcus luteus
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Staphylococcus aureus
Yes
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Other Organisms and Tissues
Sample TypePromegaExternalComments
Saccharomyces cerevisiae
Yes
A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9.
Chicken liver
Yes
Follow the standard protocol in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Miscellaneous
Sample TypePromegaExternalComments
phenol:chloroform-extracted RNA samples
Yes
A protocol for clean up for phenol:chloroform-extracted RNA samples is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Mandrekar, M. et al. (2006) Promega Notes 94, 7–11.
RNA pellet from an alcohol precipitation
Yes
A protocol for clean up of RNA pellets is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Purified RNA in an aqueous solution
Yes
A protocol for cleaning up RNA in an aqueous solution is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.

Specifications

You are viewing: Z3740 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PureYield™ Binding Columns

A245A 2 × 5 each

PureYield™ Clearing Columns

A246A 2 × 5 each

Nuclease-Free Water

P119C 1 × 25ml View Product

RNA Lysis Buffer (RLA)

Z3051 1 × 50ml

RNA Wash Solution (RWA)

Z309D 1 × 206ml View Product

RNA Dilution Buffer (RDB)

Z372A 1 × 44ml

Clearing Agent (CAA)

Z373A 1 × 11ml

Beta-Mercaptoethanol

Z523A 1 × 2ml97.4%

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: Z3741 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PureYield™ Binding Columns

A245A 10 × 5 each

PureYield™ Clearing Columns

A246A 10 × 5 each

Nuclease-Free Water

P1195 1 × 150ml

RNA Lysis Buffer (RLA)

Z305D 1 × 100ml View Product

RNA Wash Solution (RWA)

Z309D 3 × 206ml View Product

RNA Dilution Buffer (RDB)

Z372B 1 × 220ml

Clearing Agent (CAA)

Z373B 1 × 55ml

Beta-Mercaptoethanol

Z523A 1 × 2ml97.4%

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

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