Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Primer Extension System—AMV Reverse Transcriptase

Used to Quantify mRNA Transcripts and Map Transcriptional Start Sites

  • Includes control RNA and primer
  • Includes size markers ready for phosphorylation with T4 Polynucleotide Kinase


Catalog number selected: E3030

$ 227.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
Primer Extension System—AMV Reverse Transcriptase
40 reactions
$ 227.00
Your price: Log in

Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.


What's in the box?

Item Part # SizeAvailable Separately

1.2kb Kanamycin Positive Control RNA

C154A 1 × 100ng

Loading Dye

E343B 1 × 1ml

Primer Extension 2X Buffer

E349B 1 × 500μl

Primer Extension Sodium Pyrophosphate

E350B 1 × 75μl

ΦX174 DNA/HinfI Dephosphorylated Markers

E351A 1 × 500ng

Primer Extension Control Primer

E355A 1 × 50pmol

AMV Reverse Transcriptase

E357B 1 × 50u

T4 Polynucleotide Kinase

M410A 1 × 100u View Product

T4 Polynucleotide Kinase 10X Buffer

Q561A 1 × 100μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product


Choose language:

Download SDSPDF (418 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


See Protocol for detailed storage recommendations.

Let's find the product that meets your needs.

Talk to a Scientist