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Primer Extension System—AMV Reverse Transcriptase

Used to Quantify mRNA Transcripts and Map Transcriptional Start Sites

  • Includes control RNA and primer
  • Includes size markers ready for phosphorylation with T4 Polynucleotide Kinase

Size

Catalog number selected: E3030

$ 223.00
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Primer Extension System—AMV Reverse Transcriptase
40 reactions
$ 223.00
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Primer Extension System—AMV Reverse Transcriptase can be used to quantitate specific mRNA transcripts and map the start sites of transcription. An end-labeled oligonucleotide is hybridized to RNA and is used as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is related to the amount of targeted RNA.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

1.2kb Kanamycin Positive Control RNA

C154A 1 × 100ng

Loading Dye

E343B 1 × 1ml

Primer Extension 2X Buffer

E349B 1 × 500μl

Primer Extension Sodium Pyrophosphate

E350B 1 × 75μl

ΦX174 DNA/HinfI Dephosphorylated Markers

E351A 1 × 500ng

Primer Extension Control Primer

E355A 1 × 50pmol

AMV Reverse Transcriptase

E357B 1 × 50u

T4 Polynucleotide Kinase

M410A 1 × 100u View Product

T4 Polynucleotide Kinase 10X Buffer

Q561A 1 × 100μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

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Download SDSPDF (268 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

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