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HeLaScribe® Nuclear Extract in vitro Transcription System

Cell-Free System for in vitro Transcription of Eukaryotic Genes

  • Includes a CMV promoter positive-control template, rNTPs, Buffer, Stop Solution, Loading Dye and Nuclease-Free Water
  • Performance-tested with cytomegalovirus immediate early gene (CMV) promoter
  • Source of transcription factors, DNA-binding proteins and RNA processing components

Size

Catalog number selected: E3110

$ 464.00
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This product is discontinued
HeLaScribe® Nuclear Extract in vitro Transcription System
40 reactions
$ 464.00
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The most well characterized cell-free system for in vitro transcription of eukaryotic genes is derived from HeLa cell nuclei. HeLa nuclear extracts can support accurate transcription initiation by RNA polymerase II and exhibit both basal and regulated patterns of RNA polymerase transcription. The nuclear extract is also a source of transcription factors, DNA-binding proteins and the enzymatic machinery involved in RNA processing.

The HeLa Nuclear Extract included in the HeLaScribe® Nuclear Extract in vitro Transcription System is prepared by a modification of the method of Dignam et al. Extracts prepared by this method have been shown to allow transcription from the human transferrin gene promoter and the adenovirus 2 major late promoter.

The system includes all the necessary components for in vitro transcription as well as a positive control template (CMV immediate early promoter DNA), and is performance-tested with the cytomegalovirus immediate early gene (CMV) promoter. HeLaScribe® Nuclear Extract is also available separately.

Applications of the HeLaScribe® Nuclear Extract in vitro Transcription System include:

  • Analyzing basal and transcription factor-regulated transcription.
  • Assessing Promoter strength.
  • Mapping mRNA start sites.

References

  1. Dignam, J.D. et al. (1983) Nucl. Acids Res. 11, 1475–89.
  2. Sawadogo, M. and Roeder, R.G. (1985) Proc. Natl. Acad. Sci. USA 82, 4394–8.
  3. Briggs, M.R. et al. (1986) Science 234, 47–52.
  4. Bagchi, M.K. et al. (1990) J. Biol. Chem. 265, 5129–34.
  5. Carthew, R.W. et al. (1985) Cell 43, 439–48.
  6. Kristie, T.M. et al. (1989) EMBO J. 8, 4229–38.
  7. Sawadogo, M. et al. (1988) J. Biol. Chem. 263, 11985–93.
  8. Krainer, A.R. et al. (1984) Cell 36, 993–1005.

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A357A 1 × 500μl50mM

HeLaScribe® Nuclear Extract Transcription Grade

E309A 2 × 20 reactions View Product

HeLa Nuclear Extract 1X Transcription Buffer

E360A 1 × 400μl

HeLa Extract Stop Solution

E361A 1 × 15ml

HeLa Nuclear Extract Positive Control DNA

E362A 1 × 300ng View Product

Loading Dye

E371A 1 × 1ml

rATP, 100mM

E601A 1 × 15μl View Product

rUTP, 100mM

E602A 1 × 15μl View Product

rGTP, 100mM

E603A 1 × 15μl View Product

rCTP, 100mM

E604A 1 × 15μl View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

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Download SDSPDF (440 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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