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TCR Activating Antigen Stock Solution

Antigen for Use with LAG-3/MHCII Blockade Bioassay

  • Component of LAG-3/MHCII Blockade Bioassay, available as a standalone item

Size

Catalog number selected: K1201

$ 150.00
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This product is discontinued
TCR Activating Antigen Stock Solution
500μl
$ 150.00
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LAG-3 is an immune checkpoint receptor expressed on activated CD4+ and CD8+ T cells and natural killer (NK) cells and plays a critical role in regulating immune responses to tumor antigens and autoantigens. Engagement of LAG-3 by its best characterized ligand, major histocompatibility complex II (MHCII), inhibits TCR signaling, cytokine production and proliferation of activated T cells. Therapeutic antibodies designed to block the LAG-3/MHCII interaction show promising results in clinical trials for the treatment of a variety of cancers.

TCR Activating Antigen Stock Solution
(Cat.# K1201) is an antigen that is presented by MHCII on MHCII APC Cells to specifically activate the TCR on the LAG-3 Effector Cells, designed for use with the LAG-3/MHCII Blockade Bioassay, a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the LAG-3/MHCII interaction. 

For a detailed protocol for use of this product, please see Technical Manual #TM571.

The Assay Principle

The LAG-3/MHCII Blockade Bioassay is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of antibodies and other biologics targeting LAG-3. 

lag-3-mhcii-blockade-bioassay

Representation of the LAG-3/MHCII Blockade Bioassay. The bioassay consists of two cell lines, LAG-3 Effector Cells and MHCII APC Cells. When co-cultured, the LAG-3 inhibits TCR pathway-activated luminescence. The addition of anti-LAG-3 antibody blocks LAG-3 binding to MHCII, resulting in full TCR pathway activation, which can be detected in a dose-dependent manner by addition of Bio-Glo Reagent and quantitation with a luminometer.

Specifications

What's in the box?

Item Part # Size

TCR Activating Antigen Stock Solution

K120B 1 × 500μl

SDS

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Download SDSPDF (193 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Please see the Protocol for detailed storage conditions.

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