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CTLA-4 Blockade Bioassay, Cell Propagation Model

Allows Propagation and Banking of CTLA-4 Effector Cells for Use in CTLA-4 Blockade Bioassay


Catalog number selected: JA1400

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CTLA-4 Blockade Bioassay, Cell Propagation Model
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CTLA-4 (Cytotoxic T-lymphocyte-associated protein 4) is an immune checkpoint inhibitory receptor expressed on T cells and plays an important role in downregulating immune responses. The CTLA-4 Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure potency and stability of antibodies and other biologics designed to block the CTLA-4 interaction. 

The assay consists of two genetically engineered cell lines:

  • CTLA-4 Effector Cells: Jurkat T cells expressing human CTLA-4 and a luciferase reporter driven by a native promoter that responds to TCR/CD28 activation
  • aAPC/Raji Cells: Raji cells expressing an engineered cell surface protein designed to activate cognate TCRs in an antigen-independent manner and endogenously expressing CTLA-4 ligands CD80 and CD86

When the two cell types are co-cultured, CTLA-4 competes with CD28 for their shared ligands, CD80 and CD86, and thus inhibits CD28 pathway activation and promoter-mediated luminescence. Addition of an anti-CTLA-4 antibody blocks the interaction of CTLA-4 with its ligands CD80 and CD86 and results in promoter-mediated luminescence.

CTLA-4 Blockade Bioassay, Propagation Model, allows propagation and banking of the CTLA-4 Effector Cells. Bio-Glo™ Luciferase Assay System (Cat.# G7940, G7941) is the required reagent for use with CTLA-4 Blockade Bioassay, Propagation Model.


What's in the box?

Item Part # Size

CTLA-4 Effector Cells (CPM)

J146A 2 × 1ml

aAPC/Raji Cells (CPM)

J148A 2 × 1ml


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Use Restrictions

Not For Medical Diagnostic Use.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Patent Pending.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Product cannot be used for proficiency testing.

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