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Mouse ADCC Bioassay Effector Cells, Propagation Model

Allows Propagation and Banking of mFcγRIV ADCC Bioassay Effector Cells

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Catalog number selected: M1212

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Mouse ADCC Bioassay Effector Cells, Propagation Model
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Measure Activity of Mouse Antibodies that Bind and Activate mFcγRIV

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (e.g., tumor) cells for destruction by components of the cell-mediated immune system. Mouse FcγRIV (mFcγRIV) is the predominant receptor involved in ADCC in the mouse and is more closely related to human FcγRIIIa, the primary Fc receptor involved in ADCC in humans, than mFcγRIII.

The mFcγRIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcγRIV. Mouse IgG2a, and to a lesser extent IgG2b, are known to mediate ADCC through the activation of mFcγRIV. In contrast, mouse IgG1 does not bind to mFcγIV. The bioassay overcomes the limitations of more labor-intensive and highly variable primary cell assays. The bioassay workflow is simple and robust, compatible with 96-well and 384-well plate formats and, unlike traditional primary cell-based assays, provides a quantitative measure of ADCC with low variability and high accuracy.

mFcγRIV ADCC Bioassay Effector Cells, Propagation Model, allows propagation and banking of the mFcγRIV Effector Cells. Bio-Glo™ Luciferase Assay System is the required reagent for use with mFcγRIV ADCC Bioassay Effector Cells, Propagation Model.

Specifications

What's in the box?

Item Part # Size

mFcγRIV Effector Cells (CPM)

M112A 2 × 1ml

SDS

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Download SDSPDF (155 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Upon arrival, immediately transfer the cell vials to storage below –140°C (freezer or liquid nitrogen vapor phase) for long-term storage. Do not store cell vials submerged in liquid nitrogen. Do not store cell vials at –80°C because this will negatively affect cell viability and cell performance.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.

This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) discovery, development and monitoring of biologic drugs and vaccines, (3) quality assurance testing of biologic drugs and vaccines, and (4) product release assays for biologic drugs and vaccines. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the cells or genes stably transfected within the cells except that recipient may propagate and store the cells for long-term use. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all luminescence assays using this product or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

Product cannot be used for proficiency testing.

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