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ADCC Bioassay Effector Cells, F Variant, Propagation Model

Allows propagation and banking of the ADCC Bioassay, F Variant, Effector Cells

  • Easily quantitate impact of FcγRIIIa polymorphism on Fc effector function
  • Measure antibody potency and stability

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Catalog number selected: G9302

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ADCC Bioassay Effector Cells, F Variant, Propagation Model
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Determine the Effect of the Low Affinity F158 FcγRIIIa Polymorphism

The ADCC Reporter Bioassay, F Variant, provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. Specifically, primary donor PBMC or NK cells are replaced with a Jurkat cell stably expressing the low-affinity F158 human FcγRIIIa receptor and NFAT-induced luciferase. Importantly, the ADCC Reporter Bioassay, F Variant, demonstrates rank ordering of antibody activity equivalent to classic LDH release ADCC bioassays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.

ADCC Bioassay Effector Cells, F Variant, Propagation Model, allows propagation and banking of the ADCC Bioassay Effector Cells. Bio-Glo™ Luciferase Assay System is the required reagent for use with ADCC Bioassay Effector Cells, Propagation Model. Product includes two vials at 2 × 107 cells/ml and 0.65ml/vial. One vial should be thawed, propagated and cells frozen to create a cell bank. The remaining vial should be reserved as backup.

Specifications

What's in the box?

Item Part # Size

ADCC Bioassay Effector Cells, F Variant (CPM)

G930A 2 × 1ml

SDS

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Download SDSPDF (47 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

XX

Upon arrival, immediately transfer the vials of ADCC Bioassay Effector Cells and Target Cells for long-term storage below –140°C (freezer or liquid nitrogen vapor phase). The cells are sensitive, and care should be taken when handling. For safety reasons do not store cell vials submerged in liquid nitrogen.

Patents and Disclaimers

NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the recipient with a full refund.
This product may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) product release assays for biologic drugs. No other commercial use is allowed. "Commercial use" means any and all uses of this product by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the Effector Cells or the luciferase gene stably transfected within the Effector Cells, except that recipient may propagate and store Effector Cells for use in Antibody-Dependent Cell-Mediated Cytotoxicity assays. In addition, recipient must use Bio-Glo™ Luciferase Assay System purchased from Promega Corporation for all determinations of luminescence activity of this product, or contact Promega to obtain a license for use of this product with reagents other than Promega's. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

Licensed from Lonza Cologne GmbH under U.S. Pat. Nos. 7,700,357, 8,192,990 and 8,003,389, European Pat. Nos. 1297119, 1522587, 1607484 and 1741778 and other pending and issued patents.

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