The TransFast™ Transfection Reagent is composed of the synthetic cationic lipid, (+)-N,N [bis (2-hydroxyethyl)]-N-methyl-N- [2,3-di(tetradecanoyloxy)propyl] ammonium iodide and the neutral lipid, DOPE. The reagent is supplied as a dried lipid film that forms multilamellar vesicles upon hydration with water. Cationic liposomes designed for transfection, such as the TransFast™ Reagent, are more versatile than many traditional transfection methods. Advantages include flexibility in the macromolecules delivered, in vitro and in vivo applications, ability to more reproducibly transfect cells that are recalcitrant to other methods, and suitability for transient and stable transfection. Several types of macromolecule, including RNA and DNA in sizes ranging from oligonucleotides to plasmids and yeast artificial chromosomes, can be delivered to cells using liposomes.
TransFast™ Transfection Reagent is designed for nucleic acid delivery to eukaryotic cells in vitro and in vivo and performs well with many cell lines. We have found that TransFast™ Reagent performs particularly well for DNA delivery to NIH/3T3, CHO, 293, K562, PC12, Jurkat and insect Sf9 cells. The reagent is robust, requires less optimization than other systems, and allows transfection of cell types, such as primary cell cultures, that require continuous exposure to serum.
TransFast is easy to use; just resuspend the reagent in water, freeze, thaw, mix with DNA, and add to cells.