We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

TransFast™ Transfection Reagent

Fast, Efficient Transfection

  • Transfect in 1 hour (or as little as 30 minutes with certain cell lines)
  • Allows transfection of cell types that require continuous exposure to serum
  • High-efficiency transient and stable transfections

Size

Catalog number selected: E2431

$ 339.00 Your price: Log in

Add to Cart
This product is discontinued
Add to Helix
TransFast™ Transfection Reagent
1.2mg
$ 339.00
Your price: Log in

The TransFast™ Transfection Reagent is composed of the synthetic cationic lipid, (+)-N,N [bis (2-hydroxyethyl)]-N-methyl-N- [2,3-di(tetradecanoyloxy)propyl] ammonium iodide and the neutral lipid, DOPE. The reagent is supplied as a dried lipid film that forms multilamellar vesicles upon hydration with water. Cationic liposomes designed for transfection, such as the TransFast™ Reagent, are more versatile than many traditional transfection methods. Advantages include flexibility in the macromolecules delivered, in vitro and in vivo applications, ability to more reproducibly transfect cells that are recalcitrant to other methods, and suitability for transient and stable transfection. Several types of macromolecule, including RNA and DNA in sizes ranging from oligonucleotides to plasmids and yeast artificial chromosomes, can be delivered to cells using liposomes.

TransFast™ Transfection Reagent is designed for nucleic acid delivery to eukaryotic cells in vitro and in vivo and performs well with many cell lines. We have found that TransFast™ Reagent performs particularly well for DNA delivery to NIH/3T3, CHO, 293, K562, PC12, Jurkat and insect Sf9 cells. The reagent is robust, requires less optimization than other systems, and allows transfection of cell types, such as primary cell cultures, that require continuous exposure to serum.  

TransFast is easy to use; just resuspend the reagent in water, freeze, thaw, mix with DNA, and add to cells.

Specifications

What's in the box?

Item Part # Size

Nuclease-Free Water

E111B 2 × 1ml

TransFast™ Transfection Reagent

E243A 3 × 0.4mg

SDS

Choose language:

Certificate of Analysis

See all certificates for E2431 Search
View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom