pRL-TK Vector* This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pRL-TK Vector sequence reference points: Base pairs 4045 HSV TK promoter 7-759 Chimeric intron 826-962 T7 RNA polymerase Promoter (-17 to +2) 1006-1024 T7 RNA polymerase transcription initiation site 1023 Rluc reporter gene 1034-1969 SV40 late polyadenylation signal 2011-2212 Beta-lactamase (AmpR) 2359-3219 *Certain applications of this product may require licenses from others. Licensed under U.S. Pat. No. 5,292,658, 5,418,155 and other patents. 1 AGATCTAAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA 51 GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG 101 GAGGCGGCTC GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC 151 CGCGATCCTC TTAAGCACCC CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT 201 CGGCGGGTGG TAACTGGCGG GCCGCTGACT CGGGCGGGTC GCGCGCCCCA 251 GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG CGCCGCCGCG 301 GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG 351 ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC 401 TGCGGGACGA GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC 451 AAACACCAGG ACGGGGCACA GGTACACTAT CTTGTCACCC GGAGGCGCGA 501 GGGACTGCAG GAGCTTCAGG GAGTGGCGCA GCTGCTTCAT CCCCGTGGCC 551 CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT ATTTGCATGT 601 CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA 651 ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG 701 CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA 751 CCCGCTTAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG 801 CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC 851 AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC 901 TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT 951 TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA 1001 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGACTT CGAAAGTTTA 1051 TGATCCAGAA CAAAGGAAAC GGATGATAAC TGGTCCGCAG TGGTGGGCCA 1101 GATGTAAACA AATGAATGTT CTTGATTCAT TTATTAATTA TTATGATTCA 1151 GAAAAACATG CAGAAAATGC TGTTATTTTT TTACATGGTA ACGCGGCCTC 1201 TTCTTATTTA TGGCGACATG TTGTGCCACA TATTGAGCCA GTAGCGCGGT 1251 GTATTATACC AGACCTTATT GGTATGGGCA AATCAGGCAA ATCTGGTAAT 1301 GGTTCTTATA GGTTACTTGA TCATTACAAA TATCTTACTG CATGGTTTGA 1351 ACTTCTTAAT TTACCAAAGA AGATCATTTT TGTCGGCCAT GATTGGGGTG 1401 CTTGTTTGGC ATTTCATTAT AGCTATGAGC ATCAAGATAA GATCAAAGCA 1451 ATAGTTCACG CTGAAAGTGT AGTAGATGTG ATTGAATCAT GGGATGAATG 1501 GCCTGATATT GAAGAAGATA TTGCGTTGAT CAAATCTGAA GAAGGAGAAA 1551 AAATGGTTTT GGAGAATAAC TTCTTCGTGG AAACCATGTT GCCATCAAAA 1601 ATCATGAGAA AGTTAGAACC AGAAGAATTT GCAGCATATC TTGAACCATT 1651 CAAAGAGAAA GGTGAAGTTC GTCGTCCAAC ATTATCATGG CCTCGTGAAA 1701 TCCCGTTAGT AAAAGGTGGT AAACCTGACG TTGTACAAAT TGTTAGGAAT 1751 TATAATGCTT ATCTACGTGC AAGTGATGAT TTACCAAAAA TGTTTATTGA 1801 ATCGGACCCA GGATTCTTTT CCAATGCTAT TGTTGAAGGT GCCAAGAAGT 1851 TTCCTAATAC TGAATTTGTC AAAGTAAAAG GTCTTCATTT TTCGCAAGAA 1901 GATGCACCTG ATGAAATGGG AAAATATATC AAATCGTTCG TTGAGCGAGT 1951 TCTCAAAAAT GAACAATAAT TCTAGAGCGG CCGCTTCGAG CAGACATGAT 2001 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA 2051 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT 2101 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT 2151 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT 2201 ACAAATGTGG TAAAATCGAT AAGGATCCAG GTGGCACTTT TCGGGGAAAT 2251 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 2301 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 2351 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 2401 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 2451 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 2501 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 2551 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 2601 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 2651 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 2701 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 2751 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 2801 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 2851 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 2901 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 2951 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 3001 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 3051 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 3101 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 3151 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 3201 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 3251 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 3301 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 3351 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 3401 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 3451 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3501 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 3551 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 3601 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3651 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 3701 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 3751 TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3801 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 3851 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 3901 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 3951 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 4001 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGGC TCGAC Vector sequence updated 29-Feb-00.