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pmirGLO Dual-Luciferase miRNA Target Expression Vector

7841MA-W

Evaluate microRNA Activity by Inserting miRNA Target Sites 3´ of the Firefly Luciferase Gene

  • Reporter activity correlates with miRNA activity
  • Optimized luc2 reporter gene provides highest expression while the Renilla luciferase gene provides normalization
  • The moderate-strength PGK promoter provides more biologically relevant analysis not possible with strong promoters

Size

Catalog number selected: E1330

$ 395.00 Your price: Log In

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pmirGLO Dual-Luciferase miRNA Target Expression Vector
20μg
$ 395.00
Your price: Log In

The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization using the Dual-Luciferase® Assay and selection for stable cell lines.

Applications

  • miRNA detection.
  • miRNA binding site analysis.
  • Transcript stability study.
  • 3´-UTR analysis.
7827MA.eps
Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

Sequence Information

pmirGLO Vector GenBank® Accession Number FJ376737 and vector sequence text file.

7825MA.eps
Mechanism of action of the pmirGLO Vector.

Protocols

Specifications

What's in the box?

Item Part # Size

Oligo Annealing Buffer

C838A 1 x 1ml

pmirGLO Vector

E133A 1 x 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

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