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pGL2 Luciferase Reporter Vectors

Four Reporter Vectors that Contain luc, the Firefly Luciferase Gene

  • All vectors contain the firefly luciferase reporter gene, which enables sensitive and rapid quantitation of reporter activity
  • Deletions and site-directed mutations can be made directly to inserted DNAs without subcloning
  • Upstream polyadenylation signal minimizes spurious transcription of the reporter gene

Choose a vector

Size

Catalog number selected: E1641

$ 300.00 Your price: Log In

pGL2 Luciferase Reporter Vectors
Basic/20µg
$ 300.00
Your price: Log In
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The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus pyralis) luciferase, which is used to monitor transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors contain numerous features that aid in the characterization and mutagenesis of the putative regulatory sequences.

About the Vector Options

The reporter vectors are available in four options, depending on your needs. The Basic Vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. The Enhancer Vector contains the SV40 Enhancer Region for putative promoters that may need an additional regulator region. The Promoter Vector is suitable for cloning and testing putative enhancer regions. The Control Vector contains the SV40 Promoter and Enhancer Region and can be used as a normalization control for expression experiments.

0313VB05_3A-W

pGL2-Basic Vector GenBank® Accession Number X65323 and vector sequence text file.

0317VA03_3A-W

pGL2-Control Vector GenBank® Accession Number X65324 and vector sequence text file.

0314VA05_3A-W

pGL2-Enhancer Vector GenBank® Accession Number X65325  and vector sequence text file.

0316VA03_3A-W

pGL2-Promoter Vector GenBank® Accession Number X65326 and vector sequence text file.

Protocols

Specifications

You are viewing: E1641 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGL2-Basic DNA

E164A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Specifications

You are viewing: E1611 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGL2-Control DNA

E161A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Specifications

You are viewing: E1621 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGL2-Enhancer DNA

E162A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Specifications

You are viewing: E1631 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pGL2-Promoter DNA

E163A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

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