The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus pyralis) luciferase, which is used to monitor transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors contain numerous features that aid in the characterization and mutagenesis of the putative regulatory sequences.
About the Vector Options
The reporter vectors are available in four options, depending on your needs. The Basic Vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. The Enhancer Vector contains the SV40 Enhancer Region for putative promoters that may need an additional regulator region. The Promoter Vector is suitable for cloning and testing putative enhancer regions. The Control Vector contains the SV40 Promoter and Enhancer Region and can be used as a normalization control for expression experiments.