We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

pGEM®-luc DNA

Cassette Vector That is the Source of the luc Gene Encoding Firefly Luciferase

  • Use the unique restriction enzyme sites to remove the firefly luciferase gene
  • Vector is supplied with a glycerol stock of bacterial strain JM109
  • Use the reporter gene to analyze transcriptional activity, mRNA processing, protein structure/function, or label cells and viruses

Size

Catalog number selected: E1541

$ 237.00 Your price: Log in

pGEM®-luc DNA
20μg
$ 237.00
Your price: Log in

The pGEM®-luc Vector is a cassette vector designed to be a source of the luc gene encoding firefly luciferase, which is found in the pGL2 Luciferase Reporter Vectors. The plasmid is not intended for the expression of luciferase in eukaryotic or prokaryotic cells.

The pGEM®-luc Vector was constructed by positioning the luciferase gene (luc) in the center of the multiple cloning region of the pGEM®-11Zf(–) Vector, providing a number of unique restriction sites at both ends of the gene. Sites that are surrounded by parentheses are not unique, as additional sites for each also exist in the luciferase gene. Note also that using HindIII or NsiI to clone the luciferase gene will include upstream ATG codons, which may reduce the efficiency of expression in eukaryotes. The luciferase cassette does not contain the prokaryotic Shine-Delgarno sequence for bacterial expression.

References

  1. Ow, D.W. et al. (1986) Science 234, 856.
  2. de Wet, J.R. et al. (1987) Mol. Cell Biol. 7, 725–37.
  3. Wood, K.V. (1990) Promega Notes 28, 1–2.
0392VA05_3A-W

pGEM®-luc Vector GenBank® Accession Number X65316 and vector sequence text file.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

pGEM®-luc DNA

E154A 1 × 20μg

Bacterial Strain JM109

P975A 1 × 500μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for storage recommendations.

Let's find the product that meets your needs.

Talk to a Scientist

scientist-uk-stephen

Stephen

UK

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom