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pCAT™3 Vectors

Reporter Vectors that Express Chloramphenicol Acetyltransferase (CAT)

  • Offer reduced background CAT expression
  • Increased expression with more efficient poly(A) signal
  • Can produce ssDNA for sequencing and mutagenesis

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Catalog number selected: E1871

$ 286.00 Your price: Log In

pCAT™3 Vectors
Basic/20µg
$ 286.00
Your price: Log In
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The pCAT™3 Reporter Vectors provide a basis for the quantitative analysis of factors that may regulate mammalian gene expression. The redesigned backbone of the pCAT™3 Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors, the pCAT™3 Vectors contain a different polyadenylation site located 3´ of the gene. The redesigned backbone increases expression of the reporter gene, improves in vivo vector stability and provides greater flexibility in performing manipulations. The vectors offer optimal translation efficiency, and the multiple cloning sites are compatible with the pGL3 Luciferase Reporter Vectors

About the Vector Options

The reporter vectors are available in four options, depending on your needs. The Basic Vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. The Enhancer Vector contains the SV40 Enhancer Region for putative promoters that may need an additional regulator region. The Promoter Vector is suitable for cloning and testing putative enhancer regions. The Control Vector contains the SV40 Promoter and Enhancer Region and can be used as a normalization control for expression experiments.

1131VA08_5A-W

pCAT™3-Basic Vector GenBank® Accession Number U57024 and vector sequence text file.

1130VA08_5A-W

pCAT™3-Control Vector GenBank® Accession Number U57025 and vector sequence text file.

1129VA08_5A-W

pCAT™3-Enhancer Vector GenBank® Accession Number U57026  and vector sequence text file.

1132VA08_5A-W

pCAT™3-Promoter Vector GenBank® Accession Number U57027 and vector sequence text file.

Protocols

Specifications

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Item Part # Size

pCAT™3 Basic Vector

E187A 1 × 20μg

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

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Specifications

You are viewing: E1851 Change Configuration

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pCAT™3 Control Vector

E185A 1 × 20μg

SDS

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Specifications

You are viewing: E1881 Change Configuration

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pCAT™3 Enhancer Vector

E188A 1 × 20μg

SDS

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For Research Use Only. Not for Use in Diagnostic Procedures.

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You are viewing: E1861 Change Configuration

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pCAT™3 Promoter Vector

E186A 1 × 20μg

SDS

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For Research Use Only. Not for Use in Diagnostic Procedures.

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