pGL4.47[luc2P/SIE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.47[luc2P/SIE/Hygro] Vector sequence reference points: Base pairs 6129 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 SIE response element 285-409 Minimal promoter 455-485 luc2P reporter gene 518-2293 SV40 late poly(A) region 2333-2554 SV40 early enhancer/promoter 2602-3020 Synthetic hygromycin (HygR) coding region 3045-4082 Synthetic polyadenylation signal sequence 4106-4154 ColE1-derived plasmid replication origin 4478 Synthetic beta-lactamase (AmpR) coding region 5269-6129 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCAGCTTC ATTTCCCGTA 301 AATCGTCGAA GCTTCATTTC CCGTAAATCG TCGAAGCTTC ATTTCCCGTA 351 AATCGTCGAA GCTTCATTTC CCGTAAATCG TCGAAGCTTC ATTTCCCGTA 401 AATCGTCGAC TCGAGGATAT CAAGATCTGG CCTCGGCGGC CAAGCTTAGA 451 CACTAGAGGG TATATAATGG AAGCTCGACT TCCAGCTTGG CAATCCGGTA 501 CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA 551 GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA 601 AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG 651 CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT 701 CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT 751 CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG 801 CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG 851 CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT 901 GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA 951 TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC 1001 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA 1051 GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA 1101 TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG 1151 CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG 1201 CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC 1251 ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG 1301 GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA 1351 AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT 1401 TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG 1451 ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC 1501 CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA 1551 CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA 1601 GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC 1651 CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC 1701 CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC 1751 ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA 1801 GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA 1851 AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC 1901 CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG 1951 CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG 2001 AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG 2051 CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG 2101 CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG 2151 GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA GGTGGAGGAG 2201 CAGGCCGCCG GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA 2251 TAGACACCCT GCTGCTTGCG CCAGCGCCAG GATCAACGTC TAAGGCCGCG 2301 ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA 2351 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 2401 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 2451 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 2501 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT 2551 GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT TCCGCTGATC 2601 TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGC 2651 TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT 2701 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 2751 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 2801 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 2851 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 2901 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 2951 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 3001 TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGAAG 3051 AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA TCGAGAAGTT 3101 CGACAGTGTG AGCGACCTGA TGCAGTTGTC GGAGGGCGAA GAGAGCCGAG 3151 CCTTCAGCTT CGATGTCGGC GGACGCGGCT ATGTACTGCG GGTGAATAGC 3201 TGCGCTGATG GCTTCTACAA AGACCGCTAC GTGTACCGCC ACTTCGCCAG 3251 CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG TTCAGCGAGA 3301 GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC TCTCCAAGAC 3351 CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG CCGAAGCTAT 3401 GGATGCTATT GCCGCCGCCG ACCTCAGTCA AACCAGCGGC TTCGGCCCAT 3451 TCGGGCCCCA AGGCATCGGC CAGTACACAA CCTGGCGGGA TTTCATTTGC 3501 GCCATTGCTG ATCCCCATGT CTACCACTGG CAGACCGTGA TGGACGACAC 3551 CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG CTGTGGGCCG 3601 AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT CGGCAGCAAC 3651 AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG ACTGGTCCGA 3701 AGCTATGTTC GGGGACAGTC AGTACGAGGT GGCCAACATC TTCTTCTGGC 3751 GGCCCTGGCT GGCTTGCATG GAGCAGCAGA CTCGCTACTT CGAGCGCCGG 3801 CATCCCGAGC TGGCCGGCAG CCCTCGTCTG CGAGCCTACA TGCTGCGCAT 3851 CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC TTCGACGATG 3901 CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG CGGGGCCGGC 3951 ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG TATGGACCGA 4001 CGGCTGCGTC GAGGTGCTGG CCGACAGCGG CAACCGCCGG CCCAGTACAC 4051 GACCGCGCGC TAAGGAGGTA GGTCGAGTTT AAACTCTAGA ACCGGTCATG 4101 GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG 4151 TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG CCTTCAACCC 4201 AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA 4251 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC 4301 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 4351 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 4401 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 4451 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 4501 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 4551 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 4601 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 4651 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 4701 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 4751 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 4801 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 4851 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 4901 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 4951 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 5001 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 5051 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 5101 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 5151 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 5201 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC GGCCGCAAAT 5251 GCTAAACCAC TGCAGTGGTT ACCAGTGCTT GATCAGTGAG GCACCGATCT 5301 CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT CCCCGTCGTG 5351 TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA GCGCAGCAAT 5401 GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC 5451 AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC 5501 TCCATCCAGT CTATGAGCTG CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC 5551 AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT TGCTACTGGC ATCGTGGTAT 5601 CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC CCAGCGGTCA 5651 AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG TCAGCTCCTT 5701 AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA 5751 TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA 5801 TGCTTTTCCG TGACCGGCGA GTACTCAACC AAGTCGTTTT GTGAGTAGTG 5851 TATACGGCGA CCAAGCTGCT CTTGCCCGGC GTCTATACGG GACAACACCG 5901 CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA TCGTTCTTCG 5951 GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA GTTCGATATA 6001 GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG 6051 TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG 6101 AGTGCGACAC GAAAATGTTG GATGCTCAT Sequence file prepared 4-June-2012.