We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors

Cytokine Response Elements in Firefly Luciferase Reporter Vectors

  • Offer a range of response elements that are involved in cytokine signalling pathways
  • Each vector included an optimized firefly luciferase gene that codes a reporter protein with a one-hour half-life
  • Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line

Choose a response element

Size

Catalog number selected: E4041

$ 395.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
SIE/20µg
$ 395.00
Your price: Log in
Change Configuration

Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by inserting multiple repeats of a response element upstream of a minimal promoter (minP). We have designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. Proteins encoded by the RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity.

These response element vectors also have a hygromycin resistance selectable marker for use either in transient transfection experiments or for selection of a stable cell line. Some of these response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research. For investigating the cytokine signaling pathway, we have vectors available include that respond to interferon-α (INF-α) with the pGL4.45[luc2P/ISRE/Hygro] Vector, interleukin 6 (IL-6) with the pGL4.47[luc2P/SIE/Hygro] Vector, transforming growth factor β (TGF-β) with the pGL4.48[luc2P/SBE/Hygro] Vector and interleukin 3 (IL-3) with the pGL4.52[luc2P/STAT5RE/Hygro] Vector.

Learn about other signaling pathway vectors for stress pathways and additional signaling pathways.

Applications

  • Transcription regulation.
  • Virus-cell interactions.
  • Compound screening.
  • Post-translational modifications.
  • GPCR signaling.
  • Cell signaling.

Sequence Information

pGL4.45[luc2P/ISRE/Hygro] Vector GenBank® Accession Number JQ858514 and vector sequence text file.

pGL4.47[luc2P/SIE/Hygro] Vector GenBank® Accession Number JQ858512 and vector sequence text file.

pGL4.48[luc2P/SBE/Hygro] Vector GenBank® Accession Number JQ858517 and vector sequence text file.

pGL4.52[luc2P/STAT5 RE/Hygro] Vector GenBank® Accession Number JX206457 and vector sequence text file.

Activator/PathwayTranscription FactorBinding SiteVector NameExample Data
INF-α
STAT1:STAT2
Interferon Stimulated Response Element (ISRE)
pGL4.45[luc2P/ISRE/Hygro]
10694MA_440px
Representative data for pGL4.45[luc2P/ISRE/Hygro] in U2OS cells upon stimulation with IFNα. U2OS cells were transiently transfected with pGL4.45[luc2P/ISRE/Hygro] Vector and assayed in 96-well format after 16 hours stimulation with IFNα as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating the S.E.M. for three replicates. Luminescence was detected after addition of ONE-Glo® Reagent, using a GloMax® Multi+ instrument with a 0.5 second integration time.
TGF-β
SMAD3:SMAD4
SMAD Binding Element (SBE)
pGL4.48[luc2P/SBE/Hygro]
10697MA_400px
Representative data for pGL4.48[luc2P/SBE/Hygro] Vector in HEK293 cells upon stimulation with hTGF-β1. HEK293 cells were transiently transfected with pGL4.48[luc2P/SBE/Hygro] Vector and assayed in 96-well format after three hours stimulation with hTGF-β1 as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating S.E.M. for three replicates. Luminescence was detected after addition of ONE-Glo® Reagent, using a GloMax® 96 instrument with a 0.5 second integration time.
IL6
STAT3:STAT3
sis-Inducible Element (SIE)
pGL4.47[luc2P/SIE/Hygro]
10698MA_445px
Representative data for pGL4.47[luc2P/SIE/Hygro] Vector in HEK293 cells upon stimulation with IL-6. HEK293 cells were transiently transfected with pGL4.47[luc2P/SIE/Hygro] Vector and assayed in 96-well format after 24 hours stimulation with IL-6 as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating the S.E.M. for three replicates. Luminescence was detected after addition of ONE-Glo™ Reagent, using a GloMax® Multi+ instrument with a 0.5 second integration time.
IL3
STAT5:STAT5
STAT5 Response Element
pGL4.52[luc2P/STAT5 RE/Hygro]
10889MA_425px
Representative data for pGL4.52[luc2P/STAT5 RE/Hygro] Vector in Ba/F3 cells upon stimulation with mIL-3. Ba/F3 cells were transiently transfected with pGL4.52[luc2P/STAT5 RE/Hygro] Vector and assayed in 96-well format after 4 hours stimulation with mIL-3 as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating the S.E.M. for four replicates. Luminescence was detected after addition of ONE-Glo® Reagent, using a GloMax® Multi+ instrument with a 0.5-second integration time.

Specifications

You are viewing: E4041 Change Configuration

What's in the box?

Item Part # SizeConcentration
pGL4.47[luc2P/SIE/Hygro] Vector E404A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E4041

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

U.S. Pat. No. 7,728,118.

Specifications

You are viewing: E3671 Change Configuration

What's in the box?

Item Part # SizeConcentration
pGL4.48[luc2P/SBE/Hygro] Vector E367A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E3671

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

U.S. Pat. No. 7,728,118.

Licensed under U.S. Pat. No. 6,100,032.

Specifications

You are viewing: E4651 Change Configuration

What's in the box?

Item Part # SizeConcentration
pGL4.52[luc2P/STAT5 RE/Hygro] Vector E465A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E4651

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

U.S. Pat. No. 7,728,118.

Specifications

You are viewing: E4141 Change Configuration

What's in the box?

Item Part # SizeConcentration
pGL4.45[luc2P/ISRE/Hygro] Vector E414A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E4141

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

U.S. Pat. No. 7,728,118.

Let's find the product that meets your needs.

Talk to a Scientist

Elisa

Elisa

Spain