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Chroma-Luc™ Basic Vectors

Emission spectra of the Chroma-Luc™ enzymes with suggested filter windows.

Cloning Vectors That Generate Red and Green (Dual-Color) Luminescence in a Single Sample

  • Use as the experimental reporter vector with Chroma-Luc™ Control Vectors
  • Engineered genes are based on click beetle luciferase
  • Insert a region of interest into the multiple cloning site (MCS)

Choose a luciferase gene

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Catalog number selected: E1411

$ 398.00 Your price: Log in

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Chroma-Luc™ Basic Vectors
Red (CBRluc)/20µg
$ 398.00
Your price: Log in
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The pCBR-Basic, pCBG68-Basic and pCBG99-Basic Chroma-Luc™ Vectors are used for cloning regulatory elements of interest. Cells transfected with Chroma-Luc™ Vectors and used with the Chroma-Glo™ Luciferase Assay System reagent can be used to generate red and green (dual-color) luminescence from a single sample. Use the Control Chroma-Luc™ Vectors serve as an internal control for Basic Vectors.

By having two reporter signals from adding a single substrate, you can increase your accuracy and precision through normalization, or use both reporters to multiplex experimental measurements. Use filters to spectrally separate the luminescent signals. The synthetic gene design of the Chroma-Luc™ Vectors offers high expression with minimal anomalous transcription behavior. The Chroma-Luc™ genes offer ideal control or multiplexed reporter system because the high-homology red and green luciferases minimize potential RNA and protein effects on reporter expression.

Note: Use of this product requires a luminometer fitted with filters. See the Chroma-LucReporter Vectors Technical Manual #TM059 for information on recommended filter sets.

About the Synthetic Click Beetle Luciferase Genes

The Chroma-Luc™ Vectors consist of six plasmids containing synthetic versions of a red or one of two green click beetle luciferase genes; CBRluc contains a red-emitting luciferase gene, while CBG68luc and CBG99luc contain green-emitting luciferase genes. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously. Besides their different luminescence colors, the three Chroma-Luc™ genes differ as follows: CBG99luc and CBRluc possess 99% DNA and 98% protein homology and are the ideal choice for use when working with transient expression assays; CBG68luc and CBRluc possess 68.9% DNA homology while retaining a high degree of protein homology (>98%) and thus are the preferred pair for use with stable expression assays. Each of these genes is provided either in a Basic Vector configuration containing a multiple cloning site (MCS) or a Control Vector containing an SV40 promoter and enhancer. The Chroma-Glo™ Assay has a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ Luciferases, thereby enabling the processing of many plates without prior sample preparation. Two reporter gene measurements can be efficiently and reproducibly determined from each well in a typical high-throughput screen.

3951MA01_3A-W

pCBR-Basic Vector GenBank® Accession Number AY258591 and vector sequence text file

3956MA01_3A-W

pCBG68-Basic Vector GenBank® Accession Number AY258593 and vector sequence text file.

3953MA01_3A-W

pCBG99-Basic Vector GenBank® Accession Number AY258595 and vector sequence text file.

Specifications

You are viewing: E1411 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBR-Basic Vector

E141A 1 × 20μg1μg/μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. Nos. 6,387,675 and 6,552,179 and other patents pending.

Patent Pending.

U.S. Pat. No. 7,879,540 and other patents.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E1431 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBG68-Basic Vector

E143A 1 × 20μg1μg/μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. Nos. 6,387,675 and 6,552,179 and other patents pending.

Patent Pending.

U.S. Pat. No. 7,879,540 and other patents.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E1451 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBG99-Basic Vector

E145A 1 × 20μg1μg/μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. Nos. 6,387,675 and 6,552,179 and other patents pending.

Patent Pending.

U.S. Pat. No. 7,879,540 and other patents.

Certain applications of this product may require licenses from others.

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