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Steady-Glo® Luciferase Assay System

E2520

Long-Lived Luminescence for Batch Processing in Multiwell Plates

  • Stable luminescence signal with 5-hour half-life
  • No need to remove culture medium or wash cells prior to adding assay reagent
  • Less sensitive to mixing conditions in multiwell plates. Particularly useful in 384-well plates

Size

Catalog number selected: E2510

$ 105.00 Your price: Log In

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Steady-Glo® Luciferase Assay System
10ml
$ 105.00
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Precision, Reproducibility and Extended Luminescence for High-Throughput Reporter Assays

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose—providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

The Steady-Glo® assay is less sensitive to mixing conditions in multiwell plates than other assays, and is compatible with many tissue culture media, including those containing up to 10% serum.

Assay Advantages

  • No Sample Preprocessing. No need to remove culture medium or wash cells prior to adding assay reagent. Grow cells and assay them directly within the same multiwell plate.
  • High Sensitivity. The increased light output of the Steady-Glo® Assay provides improved sensitivity over other extended-lifetime firefly luciferase assay reagents.
  • Convenient and Easy to Use. To prepare the reagent, simply mix buffer with lyophilized substrate. Add the reagent directly to cells in culture, wait 5 minutes and measure luminescence.  

Learn more about custom options for this product at: www.promega.com/custom/ 
Contact Promega for information on bulk purchases.

Extended bright light output allows high-sensitivity assays to be run in batch or continuous operations.
Extended bright light output allows high-sensitivity assays to be run in batch or continuous operations.

Protocols

Specifications

You are viewing: 10ml Change Configuration

What's in the box?

Item Part # Size

Steady-Glo® Luciferase Assay Substrate

E253A 1 x 1 vial

Steady-Glo® Luciferase Assay Buffer

E254A 1 x 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

Certain applications of this product may require licenses from others.

Specifications

You are viewing: 100ml Change Configuration

What's in the box?

Item Part # Size

Steady-Glo® Luciferase Assay Substrate

E253B 1 x 1 vial

Steady-Glo® Luciferase Assay Buffer

E254B 1 x 100ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

Certain applications of this product may require licenses from others.

Specifications

You are viewing: 10 × 100ml Change Configuration

What's in the box?

Item Part # Size

Steady-Glo® Luciferase Assay Substrate

E253B 10 x 1 vial

Steady-Glo® Luciferase Assay Buffer

E254B 10 x 100ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

 

Patents and Disclaimers

Certain applications of this product may require licenses from others.

Resources

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