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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Renilla Luciferase Assay System is designed to provide a fast, sensitive method of detecting luciferase from sea pansy (Renilla reniformis). The system is a convenient alternative to firefly (Photinus pyralis) reporter systems and is designed to yield reliable, linear results for a concentration range over 7 orders of magnitude.
The Renilla Luciferase Assay System is formulated with a proprietary composition that significantly reduces the effect of coelenterazine autoluminescence compared to other reagents, making the reagent orders of magnitude more sensitive than published methods. This system enables measurements with wildtype and synthetic hRluc genes for primary expression or internal normalization measurements of gene expression.
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Renilla Luciferase Assay System Technical Manual
(711 KB pdf)
Experienced User Protocols
(100 KB pdf)
Renilla Luciferase Assay Substrate
Renilla Luciferase Assay Buffer
Renilla Luciferase Assay Lysis Buffer 5X
PDF 0 B
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U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.
Certain applications of this product may require licenses from others.
Poly(A)-binding protein modulates mRNA susceptibility to cap-dependent miRNA-mediated repression.
Simple "add-and-read" assay for Renilla luciferase reporters. Optimized for batch and continuous-processing.
E2710, E2720, E2750
Add-mix-measure assay for NanoLuc® Luciferase with >2 hour half-life in most applications.
N1110, N1120, N1130, N1150
Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample.
N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551
A sensitive reporter assay with long-lived luminescence for batch processing in multiwell plates.
E2510, E2520, E2550
Up to 15μg transfection-ready plasmid from 3ml cultures in 10 minutes.
Dye-based qPCR 2X mix for robust and sensitive amplification.
High-performance multimode system for cell health and metabolism, reporter gene activity and NGS applications.
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