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ONE-Glo™ EX Luciferase Assay System

Highly Sensitive Assay with Long-Lived Luminescence

  • Bright, stable signal that can be measured for hours
  • Stable reagent can be stored at room temperature or 4°C
  • Reduced sensitivity to culture media, phenol red and luciferase inhibitors

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Catalog number selected: E8110

$ 148.00
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ONE-Glo™ EX Luciferase Assay System
10ml
$ 148.00
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Bright, Stable, Firefly Luciferase Assay with Improved Handling and Performance

High- or ultrahigh-throughput quantitation of firefly luciferase expression in mammalian cells is commonly performed by measuring luminescence from 96-, 384- or 1,536-well plates. The ONE-Glo™ EX Luciferase Assay System provides both the high sensitivity and long-lived luminescence required to batch-process multiple plates in these assay formats.

Assay Advantages

  • Increased stability of reconstituted reagent at room temperature or 4°C simplifies repeat use over long experiments and reduces waste; no odor-causing compounds in the reagent.
  • Bright and stable signal that can be measured for hours enables more consistent luminescence readings; ideal for screening and batch processing.
  • The homogenous firefly luciferase detection reagent can be used in both single- or dual-luciferase assays; compatible with any plate size up to 1,536-well format.
  • Fewer Unwanted Effects from Sample Components. Reduced sensitivity to culture media, phenol red and luciferase inhibitors compared to other luciferase assays.
Download our Green Sheet to learn more about the features that make this an Environmentally Preferable Product:
Green Sheet

Stable Luminescence Signal Supports Batch Processing

The ONE-Glo™ EX Assay offers many of the same benefits of the ONE-Glo™ Assay, including use of  5´-fluoroluciferin substrate and an add-mix-read, or homogeneous, protocol. However, the ONE-Glo™ EX Reagent uses a new assay chemistry to increase the stability of both the luminescence signal and the reconstituted reagent. The approximately 2-hour signal half-life provides greater flexibility in assay design.

The example data shown here illustrate how the ONE-Glo™ EX signal persisted over 2 hours. In this experiment, 100µl purified firefly luciferase (13.8ng/ml) was combined in a 96-well plate with 100µl of Bright-Glo™, ONE-Glo™, ONE-Glo™ EX, Dual-Glo® Luciferase or Steady-Glo® Reagents. Luminescence was measured periodically over 2 hours, n = 8.

ONE-Glo™ EX Reagent provides bright and stable luminescence that can be measured for hours.

More Storage Options for Reconstituted Reagent

The reconstituted ONE-Glo™ EX reagent can be stored at room temperature for longer periods, so there is less variability in reagent performance during long experiments or screens and less sample waste. ONE-Glo™ EX Reagent is the firefly luciferase detection reagent used in the Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay System; the same reagent is used for single- or dual-luciferase assays.

This data shows the stability of the ONE-Glo™ EX Reagent over 20 days when stored at 22°C or 7°C.

ONE-Glo™ EX Reagent displays extended stability once reconstituted.

Specifications

You are viewing: E8110 Change Configuration

What's in the box?

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632A 1 × 10ml

ONE-Glo™ EX Luciferase Assay Substrate

E633A 1 × 1 vial

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E8120 Change Configuration

What's in the box?

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632B 1 × 100ml

ONE-Glo™ EX Luciferase Assay Substrate

E633B 1 × 1 vial

SDS

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Download SDS PDF (0 B)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E8130 Change Configuration

What's in the box?

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632A 10 × 10ml

ONE-Glo™ EX Luciferase Assay Substrate

E633A 10 × 1 vial

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for E8130

Search

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E8150 Change Configuration

What's in the box?

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632B 10 × 100ml

ONE-Glo™ EX Luciferase Assay Substrate

E633B 10 × 1 vial

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for E8150

Search

View more results

No results

Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Patent Pending.

Certain applications of this product may require licenses from others.

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