We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Luciferase Assay Reagent

Ready-to-Use Reagent for Detection of Firefly Luciferase Activity

  • Convenient premixed reagent containing Luciferase Assay Substrate and Buffer, used in the Luciferase Assay System (Cat.# E1500, E1501)
  • Linear over eight or more orders of magnitude of enzyme concentration
  • Sensitive to 10–20 moles of luciferase

Size

Catalog number selected: E1483

$ 686.00 Your price: Log In

Luciferase Assay Reagent
100ml
$ 686.00
Your price: Log In

Simple Non-Homogenous Assay to Quantify Firefly Luciferase Activity 

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10–20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so the assay is compatible with measurement of luciferase using a single-tube luminometer or a multiwell plate luminometer with an auto-injector. A luminometer is preferred, but not required as the assay is adaptable to scintillation counters.

 

Cell Lysis Reagents used with the Luciferase Assay System

The Luciferase Assay System is a nonhomogeneous assay; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay System for reporter quantitation in mammalian cells. Reporter Lysis Buffer enables luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. The Luciferase Assay System also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications.

In some kits the lysis buffer is included, and in others it must be purchased separately (see the Specifications section of this page to view assay components).

References

  1. Wood, K.V. (1991) In: Bioluminescence & Chemiluminescence: Current Status, eds. P. Stanley and L. Kricka, John Wiley & Sons, Ltd., Chichester, 543.
  2. Seliger, H.H. and McElroy, W.D. (1960) Arch. Biochem. Biophys. 88, 136.
  3. Wood, K.V. et al. (1984) Biochem. Biophys. Res. Comm. 124, 592–6.
  4. de Wet, J.R. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7870–3.
  5. Alam, J. and Cook, J.L. (1990) Anal. Biochem. 188, 245–54.
  6. Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
  7. Jones, D.P. et al. (1995) Promega Notes 54, 20–5.

Specifications

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Patents and Disclaimers

Certain applications of this product may require licenses from others.

Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom